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Biotechrabbit

[Biotechrabbit] GenUP™ PCR/Gel Cleanup Kit

Cat-No. BR0700501


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


GenUP™ PCR/Gel Cleanup Kit



제품 번호


BR0700501 - 10 preps

BR0700502 - 50 preps

BR0700503 - 250 preps


제품 특징


Features

  • Dual performance kit for both PCR product clean-up and DNA purification from agarose gels
  • Fast and simple procedure
  • High DNA recovery yields

Applications

  • Fast purification of DNA from agarose gels
  • Fast purification of products from PCR amplification reactions



Description


biotechrabbit™ GenUP PCR/Gel Cleanup Kit has been specially developed for a quick and easy cleanup or concentration of PCR fragments from reaction mixtures as well as extraction of DNA from both TAE and TBE agarose gels. The DNA is bound to a Mini Filter using a novel buffer, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including restriction digestion, ligation, sequencing, transfection into mammalian cells and in vitro transcription.



Other Information


STARTING MATERIAL

PCR reaction mixtures (up to 50 µl)

TAE or TBE agarose gel (up to 300 mg)

EXTRACTION TIME

Approximately 3 min

Approximately 20 min

BINDING CAPACITY

> 20 µg DNA

> 20 µg DNA

DNA SIZE

60 bp – 30 kb

100 bp – 30 kb

RECOVERY RATE

60–95%

60–90%



Protocols

STEPS

  • PCR products only: Transfer the PCR sample to a centrifugation tube, add Buffer BINDING BP and mix well.
  • Agarose gel only: Cut the gel slice containing the DNA fragment, transfer to a reaction tube. Add Buffer DISSOLVING and incubate.
  • Transfer the sample to the Mini Filter (green) placed in a Collection Tube and centrifuge.
  • Wash Mini Filter with Buffer WASH B and centrifuge.
  • Add Buffer ELUTION and centrifuge.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).









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