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Biotechrabbit

[Biotechrabbit] GenUP™ Exo SAP Kit

Cat-No. BR0701801


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GenUP™ Exo SAP Kit



제품 번호


BR0701801


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Features

  • Fast 15 min PCR cleanup without the need for spin columns
  • 100% recovery — no loss of PCR product
  • Simple process — just add Exonuclease 1 and Shrimp Alkaline Phosphatase to PCR mix
  • Easy scalability and automation

Applications

  • PCR cleanup prior to sequencing or genotyping
  • Removal of nucleotides and primers from PCR reaction



Description


biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.

The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Exonuclease 1 (ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.

Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3' → 5' exonuclease activity of Exonuclease 1 degrades single-stranded primers.



Other Information

Component

Composition

ExoI-SAP

Exonuclease I and SAP in storage buffer containing 50% glycerol (v/v).

 

STORAGE

−20°C (until expiry date – see product label)

 

Quality Control
Shrimp Alkaline Phosphatase
Specificity

SAP Removes 5’-phosphates from DNA, RNA, dNTPs and proteins.

Purity

Purified to apparent homogeneity. SAP is highly pure and tested free of contaminating nucleases.

Exonuclease 1
Specificity

ExoI is a 3’-5’ exonuclease, specific for single stranded DNA.

Purity

Purified to apparent homogeneity. ExoI is highly pure and tested free of contaminating nucleases.



Protocols

STEPS

  • Add 2 µl ExoI-SAP to 5 µl of a completed PCR reaction.
  • Incubate at 37°C for 5 min.
  • Heat inactivate at 80°C for 10 min.

For detailed protocol, please, refer to the product manual (Related Documents).



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