Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.
제품 설명
GenUP™ Plasmid Kit
제품 번호
BR0700201 - 10 preps
BR0700202 - 50 preps
BR0700203 - 250 preps
제품 특징
Features
- Universal kit for both high- and low-copy-number plasmids isolation
- Fast and simple procedure
- High yields of pure plasmid DNA suitable for all applications
Applications
- Plasmid DNA isolation from Gram-negative and Gram-positive bacteria
Description
biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.
The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
Other Information
STARTING MATERIAL | Bacterial cultures: 0.5–15 ml for high-copy-number plasmids, low-copy-number plasmids or cosmids | EXTRACTION TIME | Approximately 15 min | BINDING CAPACITY | Approximately 80 µg plasmid DNA | TYPICAL YIELD | Variable: 45–60 µg high-copy plasmid DNA is typically purified from 15 ml culture, or 6–20 µg from 2 ml culture | AVERAGE PURITY | A260/A280 1.7–2.0 |
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Protocols
STEPS | - Pellet bacterial culture and centrifuge. Remove the supernatant.
- Resuspend the cell pellet in Buffer RESUSPENSION and vortex.
- Optional: Pre-treatment of Gram-positive bacteria with lysozyme.
- Add Buffer LYSIS LP, mix and incubate.
- Add Buffer NEUTRALIZATION, mix and centrifuge.
| - Transfer lysate to Mini Filter (orange) and centrifuge.
| - Wash with Buffer WASH PA and Buffer WASH PB and centrifuge.
- Centrifuge again to remove residual ethanol.
| - Elute DNA with Buffer ELUTION EP.
- Purified DNA in the Elution Tube is ready for use.
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For detailed protocol, please, refer to the product manual (Related Documents).
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