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Biotechrabbit

[Biotechrabbit] GenUP™ Blood DNA Kit

Cat-No. BR0701301


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



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GenUP™ Blood DNA Kit



제품 번호


BR0701301 - 10 preps

BR0701302 - 50 preps

BR0701303 - 250 preps


제품 특징


Features

  • Fast and simple procedure
  • Genomic gDNA from fresh and frozen, EDTA- or citrate-treated blood
  • Excellent genomic DNA quality in yields of up to 30 µg

Applications

  • Isolation of genomic DNA from up to 400 µl whole blood



Description


biotechrabbit™ GenUP Blood DNA Kit is designed for fast isolation of genomic DNA from up to 400 µl whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. After an efficient lysis step, genomic DNA is bound to a Mini Filter, washed and eluted. The isolation chemistry and extraction protocol are optimized for maximum yield. Including lysis, isolated DNA is available in approximately 24 min. The isolated DNA is suitable for a wide range of different molecular biology applications.

Protocols are available for isolating DNA from 200 µl or 400 µl whole blood samples.

The GenUP Blood DNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP gDNA Kit (cat. no. BR0700601), GenUP Bacteria gDNA Kit (cat. no. BR0700701), GenUP Plant DNA Kit (cat. no. BR0700801) or GenUP Virus DNA/RNA Kit (cat. no. BR0701101).



Other Information

STARTING MATERIAL

Fresh or frozen whole blood; stabilized with EDTA or citrate (200 µl or 400 µl)

EXTRACTION TIME

Approximately 24 min

BINDING CAPACITY

> 60 µg DNA

TYPICAL YIELD

Variable depending on the starting material; approximately 30 µg DNA

AVERAGE PURITY

A260/A280 1.7–2.0


Protocols

STEPS

  • If necessary, add PBS to the samples for a volume of 200 µl or 400 µl.
  • Add Buffer LYSIS LC and Proteinase K and incubate.
  • Add Buffer BINDING BL and mix well.
  • Transfer the sample to the Mini Filter (red) placed in a Collection Tube and centrifuge.
  • Add Buffer WASH WA and centrifuge.
  • Add Buffer WASH WB and centrifuge.
  • Centrifuge again to remove the wash buffer.
  • Elute DNA with Buffer ELUTION and centrifuge.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).



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