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Biotechrabbit

[Biotechrabbit] GenUP™ Plant DNA Kit

Cat-No. BR0700801


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


GenUP™ Plant DNA Kit



제품 번호


BR0700801 - 10 preps

BR0700802 - 50 preps

BR0700803 - 250 preps


제품 특징


Features

  • Simple and efficient procedure for plant DNA isolation
  • Special lysis protocols for different plant materials
  • High yields of inhibitor-free DNA

Applications

  • Universal kit for isolating genomic DNA from various plant materials



Description


biotechrabbit™ GenUP Plant DNA Kit has been specially developed for quick and easy purification of genomic DNA from a wide variety of plant materials, including fresh, frozen or dried samples from leaves, roots, stems and flowers.

The kit includes an advanced prefiltration step to remove unlysed tissue. Subsequently, DNA is bound to a Mini Filter and is subsequently washed and eluted in a separate tube. The purified DNA is ready for use in any demanding molecular biology application, including PCR, enzymatic digestions and cloning.

This kit provides three buffers for optimized processing with different plant materials. To determine optimal lysis conditions, side-by-side preparation using the three provided protocols are prepared.



Other Information

STARTING MATERIAL

Fresh, frozen or dried plant tissue (maximum 100 mg dry weight
or 180 mg wet weight)

EXTRACTION TIME

Approximately 40 min

BINDING CAPACITY

> 50 µg DNA

TYPICAL YIELD

Variable depending on the starting material; approximately 5–25 µg DNA

AVERAGE PURITY

A260/A280 1.7–2.0


Protocols

STEPS

  • Homogenize plant material.
  • Mortar and pestle in presence of liquid nitrogen.
  • Homogenizer, e.g., rotor-stator or bead mill.
  • Lysis dependent on Buffer LYSIS:
    • add Buffer LYSIS LC, incubate and proceed with the next step.
    • add Buffer LYSIS LS or Buffer LYSIS LD, incubate, add Buffer PRECIPITATION and centrifuge to remove unlysed material.
  • Filter lysate using a Pre Filter (lavender).
  • Bind DNA to the Mini Filter (green) with Buffer BINDING BD.
  • Centrifuge to remove unbound material.
  • Wash the DNA twice with Buffer WASH C.
  • Centrifuge twice to remove the wash buffer.
  • Elute DNA with Buffer ELUTION and centrifuge.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).



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Biotechrabbit - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"

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