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Biotechrabbit

[Biotechrabbit] GenUP™ Total RNA Kit

Cat-No. BR0700901


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


GenUP™ Total RNA Kit



제품 번호


BR0701801 - 10 preps

BR0701802 - 50 preps

BR0701803 - 250 preps


제품 특징


Features

  • Fast and simple procedure
  • High yields of pure RNA
  • Physical removal of DNA, no DNase treatment, no toxic β‑mercaptoethanol

Applications

  • Universal kit for total RNA isolation from various sources and different amounts of starting material



Description


biotechrabbit™ GenUP Total RNA Kit has been specially developed for a quick and easy purification of total RNA from eukaryotic cell suspensions, tissues and biopsies, Gram-negative (e.g., E. coli) and Gram-positive bacteria and other sources. After few initial procedures, the RNA is bound to a filter, washed and then eluted in a separate tube. DNA is removed physically by binding to a filter without any DNAse treatment or the use of toxic β‑mercaptoethanol. The purified RNA is ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis and others.



Other Information

STARTING MATERIAL

Eukaryotic cells (5 × 106), tissue samples (up to 20 mg), bacterial cells
(Gram-positive or Gram-negative, 1 × 109)

EXTRACTION TIME

Approximately 20–40 min

BINDING CAPACITY

100 µg RNA

TYPICAL YIELD

Yield is highly dependent on sample type


Protocols

STEPS

  • Lyse the sample material.
  • Centrifuge to pellet unlysed material.
  • Transfer the lysate to the Mini Filter DNA (blue).
  • Centrifuge, and discard the Mini Filter DNA.
  • The filtrate contains the RNA, do not discard.
  • Add ethanol to the filtrate.
  • Transfer to a Mini Filter RNA (violet) to bind RNA and centrifuge.
  • Add Buffer WASH A and centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Add Buffer Water, RNase-free (for ELUTION), incubate and centrifuge.
  • RNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).



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