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Biotechrabbit

[Biotechrabbit] TriColor Broad Protein Ladder (3.5-245 kDa)

Cat-No. BR0900101


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


TriColor Broad Protein Ladder (3.5-245 kDa)



제품 번호


BR0900101


제품 특징


Features

  • Ready-to-use prestained protein ladders covering 3.5–245 kDa ranges
  • Pure and stable — retain sharp bands after 3 months storage at 4°C
  • Protein bands in three colors with easily recognizable colored reference bands

Applications

  • Approximate protein sizing on SDS PAGE and western blots
  • Monitoring protein gel electrophoresis and western transfers



Description


biotechrabbit™ TriColor Broad Protein Ladder facilitates approximate molecular-weight estimation of proteins on denaturing polyacrylamide gels, monitoring protein separation during electrophoresis and verification of western transfer efficiency to membranes (polyvinylidene difluoride, nylon or nitrocellulose). The ladder provides three colored reference bands.

TriColor Broad Protein Ladder is ready for immediate use and is notably stable: sharp bands are produced after storage for 2 weeks at room temperature or 3 months at 4°C, eliminating the need to thaw before loading.



Other Information


Component

Composition

TriColor Broad Protein Ladder (3.5–245 kDa)

Ready-to use protein ladder containing 0.1 – 0.4 mg/ml of each protein in 20 mM Tris-phosphate, pH 7.5, 2 % SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol.

 

STORAGE

−20°C (until expiry date – see product label)

 

Quality Control
Functional assay

Appearance of all stated bands tested in SDS-Polyacrylamide gel electrophoresis.




Protocols

Component

LOADING VOLUME

ACTIONS BEFORE LOADING

TriColor Broad Protein Ladder (3.5–245 kDa)

3 or 5 µl for 15 or 10-well mini-gel, respectively.

1.5 or 2.5 µl for Western transfer.

Apply more for thicker (>1.5 mm) or larger gels.

Direct loading




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