Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.
제품 설명
Proteinase K
제품 번호
BR1100901 - 30mg
BR1100902 - 150mg
BR1101001 - 1.25ml
BR1101002 - 10ml
제품 특징
Description
biotechrabbit™ Proteinase K is an active endopeptidase that is effective with native proteins, allowing endogenous RNases and DNases to be inactivated rapidly. The robust enzyme is stable over a wide pH range (4–12.5) and remains fully active for several hours when incubated at pH 6.5–9.5. The exceptional purity of the enzyme ensures that it is ideally suited for preparing PCR templates, as it is free of RNases, DNases and DNA.
biotechrabbit Proteinase K is available as an aqueous solution or lyophilized powder.
Other Information
StorageStore lyophilised powder at 4°C or room temperature. Store solution at 4°C. Quality ControlProtein ConcentrationThe protein concentration of soluble Proteinase K is analyzed by Biuret assay (20 mg, +/- 2 mg). Protease activityThe activity is analyzed with hemoglobin as substrate at 37°C (>600 U/ml). Absence of nuclease activityThe enzyme is tested for absence of endonuclease and nicking activities. RNase contaminationThe enzyme is tested for absence of RNase activity. DNA contaminationThe enzyme is tested for DNA (threshold 10 pg/mg). BioburdenThe enzyme is tested for biodurden (<1 CFU/ml). |
Protocols
Inhibitors
The enzyme is not inactivated by metal ions, chelating agents (such as EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.
Activators
The Proteinase K activity is stimulated by denaturing agents (SDS and urea). The presence of SDS can produce a seven-fold increase in Proteinase K activity.
pH Optimum
Proteinase K is stable over a broad pH range (4 – 12.5). The enzyme retains its full activity for several hours when incubated at a pH between 6.5 and 9.5.
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