Synbio Technologies는 혁신적인 합성 생물학 솔루션을 제공하여 생명과학 연구 및 개발을 지원하는 기업입니다.

The General Introduction of Gene Shuttle Vector

Synbio Technologies is excited to introduce our gene shuttle vector related services! Our modified shuttle vector enables protein expression detection without requiring transformation operations.

Compared to existing expression vectors in the market, our shuttle expression vector offers enhanced efficiency and reliability. The vector streamlines experimental procedures, which significantly reduces complexity. Additionally, it’s high-efficiency dual-system compatibility enhances expression capabilities. When used in combination with our ProXpress rapid protein detection kit, you can preview the expression of synthesized plasmids before receiving them - accelerating the progress of your scientific research!

Highlights

Highly efficient and stable protein expression in eukaryotic and prokaryotic systems.

No need to transform expression strains such as BL21 (DE3), shortening the verification cycle.

Instant protein expression detection with ProXpress.

Customized vector transformations available to meet specific research needs.

Service Details

* Turnaround time applies to gene synthesis of non-complex sequences. For gene synthesis of complex sequences, please consult: quote@synbio-tech.com.

** Optional protein validation with ProXpress for $35 each.

Workflow

Deliverables

Case Study

1. Background

The shuttle vector expressing green fluorescent protein (GFP) wastransferred into prokaryotic cells ( Mach1-T1 ).

The results show that

• The supernatant of the bacterial lysate was observed under ultraviolet (UV) light and green fluorescence was detected.

• Using the Flag-tag protein rapid detection card,protein expression was confirmed10 minutes later,showing high levels of proteinexpression.

2. Background

Aconstructed shuttle expression vector was transfected into HEK 293 cell and compared witha conventional expression vector.

The results show that

Comparedto the conventional vector, the modified shuttle expression vectorshowed significantlyhigherproteinexpression in HEK 293 cell.

FAQs

What are the possible reasons for failed gene cloning when inserting a target gene into a shuttle vector?

Possible causes include:

1. Unreasonable primer design
2. Poor template quality
3. Incorrect PCR program settings.

Compared to conventional vectors, what advantages do shuttle vectors offer?

1. Dual system compatibility: Shuttle vectors contain two or more replicons from different genetic backgrounds,enabling replication in two or more different organisms.
2. Selective markers: Shuttle vectors usually contain selective markers, such as antibiotic resistance genes, allowing for easy screening of clones containing the target gene via antibiotic selection.
3. Higher Efficiency: Shuttle vectors are typically more efficient when introduced into the target biological system.

What are the application areas of shuttle vectors?

Shuttle vectors are used in various fields, including:

1. Gene function research
2. Protein interaction analysis
3. Drug target screening
4. Vaccine development

The efficiency of shuttle vectors makes them suitable for various application, ranging from academic research to drug development,supporting scientists and researchers in their pursuit of research and innovation.

SYNBIO의 모든 제품을 만나 보세요!

SERVICES

DNA Synthesis

Vector Selection

Molecular Biology

Oligo Synthesis

RNA Synthesis

Variant Libraries

CRISPR Libraries

Oligo Pools

Virus Packaging

Gene Editing

Protein Expression

Antibody Services

Peptide Services

DNA Data Storage

PRODUCTS

Standard Oligo

Standard CRISPR Libraries

Standard CRISPR Plasmid

ProXpress

Protein Products

ONE STOP SOLUTION

High Performing DNA-RNA-Protein Molecules

Oligonucleotide Diagnostics & Therapeutics

Precision Gene Editing

Value Chain

SYNBIO Technologies - Exclusive Distributor in South Korea "Morebio" 한국 독점 대리점 "모아바이오"