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The General Introduction of Recombinant Collagen Gene Synthesis
The correct amino acid sequence is essential for the proper function of collagen. In the preparation of triple helix peptides with specific collagen sequences, the main technical difficulties lie in the selection of gene fragments and the stability of the triple helix structure.
Synbio Technologies has an advanced synthetic bio-empowering technology platform to synthesize recombinant collagen “high” repetitive genes with leading-edge design and advanced manufacturing. Customized gene synthesis design, high-qualityE. coliengineering strain libraries, and a complete molecular cloning tool enzyme system can help you solve the difficulties of recombinant collagen gene synthesis. We can assist you in experimenting with many gene designs in the lab at a very low cost, while improving the expression, hydrophilicity, and processability of the collagen – unlocking countless application possibilities.
Highlights
Gene sequences are 100% accurate
Customized gene synthesis protocol design
High quality engineering strain library
Complete molecular cloning tool enzyme system
Service Details
Deliverables
2-5 μg Lyophilized Powder
COA
Sequencing Report
Case Study
Recombinant Collagen Gene Synthesis
In this case, Synbio Technologies successfully synthesized and delivered 100% accurate collagen high-repetitive gene sequences. We independently developed a variety of biological intelligent analysis tools such as Complexity Index (CI), NG Codon Optimization, AI-TAT, DNA Studio, etc., to quickly design and assemble genes. A high-quality strain library and a complete molecular cloning tool enzyme system are combined with our own patented reagents Syno Interlinkage and Syno Assembly to help you accelerate the optimization and application development of recombinant collagen production from the genetic source.
FAQs
What are the main technical difficulties in recombinant collagen gene synthesis?
Gene Fragment Selection: Since collagen is a very large protein molecule with a molecular weight of up to 300kDa, its gene sequence is complex and contains multiple repeat units (such as periodic arrangement of (Gly-X-Y) n). In the process of gene synthesis, the correct selection and combination of these gene fragments to ensure the correct expression and function of collagen is crucial.
Triple Helix Structure Stability: The triple helix structure of collagen is the key to its stability and function. However, ensuring that collagen can correctly fold to form a stable triple helix structure during gene synthesis and expression is a difficult challenge.
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