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Synbio Technologies

[Synbio Technologies] Plasmid DNA Preparation

Cat-No.


Synbio Technologies는 혁신적인 합성 생물학 솔루션을 제공하여 생명과학 연구 및 개발을 지원하는 기업입니다.




The General Introduction of Plasmid DNA Preparation

Plasmid DNA preparation is a process of separating and purifying circular double-stranded DNA molecules (i.e., plasmids) from bacteria (usually Escherichia coli) that contain plasmids. As small DNA fragments independent of chromosomal DNA and capable of autonomous replication in host cells, plasmids often carry genes such as antibiotic resistance genes and fluorescent protein genes, which endow host cells with specific biological characteristics. It is extremely useful in molecular biology research and biotechnology fields. Its main purpose is to provide materials for subsequent experiments such as cloning, sequencing, or serving as a vector for transforming other cells.

 

Synbio Technologies is dedicated to providing our customers with high-quality and customized plasmid DNA preparation services. We conduct comprehensive quality control of the plasmid products to provide you with aseptic plasmids containing no RNA or genome pollution. All plasmids we manufacture are free of animal-derived materials and can contain low levels of endotoxin (< 100 EU/mg, <30 EU/mg, <5 EU/mg, on request). We provide both research grade and transfection grade plasmids according to our customers needs. Order your plasmid DNA now and experience something brand new right away!

 

Highlights

Fast Turnaround Time

As fast as 2 BDs

Guaranteed Plasmid Preparation Services

USA-based manufacturing ensures high quality products

Highly Customized

Microgram-to-gram-scale quantity can meet various customer needs

Low Endotoxin Level

Animal-derived materials free, <100 EU/mg, on request

Strict Quality Control

ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation

One-stop Service

We provide a one-stop solution from gene synthesis to plasmid production

 

Service Details

Service Specifications

 

SizeTATEndotoxin LevelPrice
Research Grade100-200 μg5-8 BD< 0.1 EU/μg
<0.05 EU/μg
Quote
200-500 μg5-8 BD
500-1000 μg5-8 BD
HT Grade15 μg5-8 BD
GMP-Like Grade1-10 mg6-9 BD
10-50 mg9-13 BD
50 mg-1 gQuote
GMP Grade100 mg-10 gQuote< 0.01 EU/μg



Quality Control

 

C Items*MethodSpecificationsDefault QC
Research Grade
Default QC
Transfection Grade
QC Price
AppearanceVisual inspectionClear, colorless, free from visible particulates.Free
A 260/280 RatioUV Absorbance1.80 ~ 2.00.Free
QuantityUV AbsorbanceQuantity is ± 5%.Free
Residual RNAAgarose gel electrophoresisNot visibleFree
Genomic DNAAgarose gel electrophoresisNot visibleFree
Fragment SizeRestriction DigestionThe size of plasmids fragment is right and free of any contaminated bands.Free
Endotoxin controlTAL assay< 100 EU/mgxFree
Supercoil contentAgarose gel electrophoresisSupercoil content >90%xFree
BioburdenStreak inoculationNo growth after 48 hours.xFree
MycoplasmaStreak inoculationNo mycoplasma is detected in the plasmid products.xxQuote
SequencingWhole plasmids sequencingSequencing results are consistent with the confirmed plasmid sequence.xxQuote



*Contact us for more QC items.


Plasmid DNA Applications and Major User Groups

Plasmid DNA plays an important role in various scientific and industrial fields and is an essential tool for advances in gene therapy, diagnostics and drug development.

The major users of plasmid DNA services include researchers, R&D managers and professionals in the fields of gene therapy, in vitro diagnostics, biopharmaceuticals, industrial biotechnology and academic research.

Whether for gene therapy diagnostics, vaccine research or drug screening, plasmid DNA remains an indispensable tool for advancing medicine and science.

 

Workflow


Deliverables

Plasmid DNA (Dry powder/liquid)

 COA file

 Endotoxin test report (Optional)

 Restriction enzyme digestion analysis (Optional)

 Sequencing verification (Optional)

 

FAQs

What should I do if the plasmid copy number is low?

You can increase the volume of bacterial solution extracted and reduce the volume during elution, or replace it with a high-copy-number vector with the same function.

 

What are the downstream applications of plasmid DNA?

Our services cater to a wide range of applications, including gene function research, protein production, gene therapy, vaccine development, drug screening, and more.

 

Can you provide transfection level plasmid DNA?

We provide high-quality plasmid DNA for various applications, including transfection, and our plasmid DNA preparation services can be customized to meet your specific needs. We offer both research-grade and transfection-grade plasmid DNA. Whether you need plasmid DNA for gene delivery, cloning, or gene expression studies, we have reliable plasmid DNA preparation solutions to ensure the best possible results.

 




SYNBIO의 모든 제품을 만나 보세요!


SERVICES


DNA Synthesis

Vector Selection

Molecular Biology

Oligo Synthesis

RNA Synthesis

Variant Libraries

CRISPR Libraries

Oligo Pools

Virus Packaging

Gene Editing

Protein Expression

Antibody Services

Peptide Services

DNA Data Storage


PRODUCTS


Standard Oligo

Standard CRISPR Libraries

Standard CRISPR Plasmid

ProXpress

Protein Products


ONE STOP SOLUTION


High Performing DNA-RNA-Protein Molecules

Oligonucleotide Diagnostics & Therapeutics

Precision Gene Editing

Value Chain



SYNBIO Technologies - Exclusive Distributor in South Korea "Morebio" 한국 독점 대리점 "모아바이오"