Synbio Technologies는 혁신적인 합성 생물학 솔루션을 제공하여 생명과학 연구 및 개발을 지원하는 기업입니다.
The General Introduction of Plasmid DNA Preparation
Plasmid DNA preparation is a process of separating and purifying circular double-stranded DNA molecules (i.e., plasmids) from bacteria (usually Escherichia coli) that contain plasmids. As small DNA fragments independent of chromosomal DNA and capable of autonomous replication in host cells, plasmids often carry genes such as antibiotic resistance genes and fluorescent protein genes, which endow host cells with specific biological characteristics. It is extremely useful in molecular biology research and biotechnology fields. Its main purpose is to provide materials for subsequent experiments such as cloning, sequencing, or serving as a vector for transforming other cells.
Synbio Technologies is dedicated to providing our customers with high-quality and customized plasmid DNA preparation services. We conduct comprehensive quality control of the plasmid products to provide you with aseptic plasmids containing no RNA or genome pollution. All plasmids we manufacture are free of animal-derived materials and can contain low levels of endotoxin (< 100 EU/mg, <30 EU/mg, <5 EU/mg, on request). We provide both research grade and transfection grade plasmids according to our customers needs. Order your plasmid DNA now and experience something brand new right away!
Highlights
Fast Turnaround Time
As fast as 2 BDs
Guaranteed Plasmid Preparation Services
USA-based manufacturing ensures high quality products
Highly Customized
Microgram-to-gram-scale quantity can meet various customer needs
Low Endotoxin Level
Animal-derived materials free, <100 EU/mg, on request
Strict Quality Control
ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation
One-stop Service
We provide a one-stop solution from gene synthesis to plasmid production
Service Details
Size | TAT | Endotoxin Level | Price | |
---|---|---|---|---|
Research Grade | 100-200 μg | 5-8 BD | < 0.1 EU/μg <0.05 EU/μg | Quote |
200-500 μg | 5-8 BD | |||
500-1000 μg | 5-8 BD | |||
HT Grade | 15 μg | 5-8 BD | ||
GMP-Like Grade | 1-10 mg | 6-9 BD | ||
10-50 mg | 9-13 BD | |||
50 mg-1 g | Quote | |||
GMP Grade | 100 mg-10 g | Quote | < 0.01 EU/μg |
C Items* | Method | Specifications | Default QC Research Grade | Default QC Transfection Grade | QC Price |
---|---|---|---|---|---|
Appearance | Visual inspection | Clear, colorless, free from visible particulates. | √ | √ | Free |
A 260/280 Ratio | UV Absorbance | 1.80 ~ 2.00. | √ | √ | Free |
Quantity | UV Absorbance | Quantity is ± 5%. | √ | √ | Free |
Residual RNA | Agarose gel electrophoresis | Not visible | √ | √ | Free |
Genomic DNA | Agarose gel electrophoresis | Not visible | √ | √ | Free |
Fragment Size | Restriction Digestion | The size of plasmids fragment is right and free of any contaminated bands. | √ | √ | Free |
Endotoxin control | TAL assay | < 100 EU/mg | x | √ | Free |
Supercoil content | Agarose gel electrophoresis | Supercoil content >90% | x | √ | Free |
Bioburden | Streak inoculation | No growth after 48 hours. | x | √ | Free |
Mycoplasma | Streak inoculation | No mycoplasma is detected in the plasmid products. | x | x | Quote |
Sequencing | Whole plasmids sequencing | Sequencing results are consistent with the confirmed plasmid sequence. | x | x | Quote |
Plasmid DNA Applications and Major User Groups
Plasmid DNA plays an important role in various scientific and industrial fields and is an essential tool for advances in gene therapy, diagnostics and drug development.
The major users of plasmid DNA services include researchers, R&D managers and professionals in the fields of gene therapy, in vitro diagnostics, biopharmaceuticals, industrial biotechnology and academic research.
Whether for gene therapy diagnostics, vaccine research or drug screening, plasmid DNA remains an indispensable tool for advancing medicine and science.
Workflow
Deliverables
Plasmid DNA (Dry powder/liquid)
COA file
Endotoxin test report (Optional)
Restriction enzyme digestion analysis (Optional)
Sequencing verification (Optional)
FAQs
What should I do if the plasmid copy number is low?
You can increase the volume of bacterial solution extracted and reduce the volume during elution, or replace it with a high-copy-number vector with the same function.
What are the downstream applications of plasmid DNA?
Our services cater to a wide range of applications, including gene function research, protein production, gene therapy, vaccine development, drug screening, and more.
Can you provide transfection level plasmid DNA?
We provide high-quality plasmid DNA for various applications, including transfection, and our plasmid DNA preparation services can be customized to meet your specific needs. We offer both research-grade and transfection-grade plasmid DNA. Whether you need plasmid DNA for gene delivery, cloning, or gene expression studies, we have reliable plasmid DNA preparation solutions to ensure the best possible results.
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SERVICES
PRODUCTS
ONE STOP SOLUTION
High Performing DNA-RNA-Protein Molecules
Oligonucleotide Diagnostics & Therapeutics
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