Synbio Technologies는 혁신적인 합성 생물학 솔루션을 제공하여 생명과학 연구 및 개발을 지원하는 기업입니다.
The General Introduction of Modified Oligos
Synbio Technologies provides high-quality oligo synthesis and modification. Our specialized services include oligonucleotides (which can be modified with special bases), dU/dI, phosphorothioates, phosphorylation, spacers, amino linkers, disulfide linkers, biotin, etc. In addition, Synbio Technologies also provides multiple fluorescent groups and quenchers at 3’, 5’, or designated sites specified by the customer. Some of these sites include FAM, HEX, ROX, BHQ, Texas Red, and others. The resulting oligos can be used in a variety of applications including PCR cloning, sequence verification, and gene detection.
Highlights
Comprehensive Modifications and Labeling
The average mutation rate is lower than 1/1000
Various Modification/Labeling
Modification/labeling type 200+
Strict Quality Control
Stringent ISO9001 and ISO13485 Certified Processing for oligo synthesis
Multiple Purification Options
Includes desalt, PAGE Plus, PAGE, RP-HPLC, IE-HPLC and Dual HPLC
Service Details
General Modifications
Synbio Technologies provides a series of general modifications with high purity and competitive prices. We accomplish this by focusing on requests of modification with special chemical classifications. Browse general modifications listed below:
| Classification | Modifications | Purification |
|---|---|---|
| Modified Bases | dU | HPLC/PAGE |
| dI | ||
| 5-Methyl dC | ||
| DBCO | 5’ DBCO | |
| 3’ DBCO | ||
| dT-DBCO | ||
| AMCA | 5’ AMCA | |
| 3’ AMCA | ||
| dT-AMCA | ||
| Phosphorothioates | SPO3 | |
| Amino Linkers | 5’Amino C6 | |
| dT-Amino C6 | ||
| 3’Amino C7 | ||
| 5’Amino C12 | ||
| Biotin | 5’Biotin | |
| 3’Biotin | ||
| dT-Biotin | ||
| Digoxigenin | 5’Digoxigenin | |
| 3’Digoxigenin | ||
| dT-Digoxigenin | ||
| Phosphorylation | 5’ PO4 | |
| 3’ PO4 | ||
| Hydrosulphonyl | 5’ HS-C6 | |
| 3’ HS-C3 | ||
| Locked Nucleic Acid | LNA (A/G/C/U) | |
| Fluoro Bases | 2’-F-2’-dA | |
| 2’-F-2’-dC | ||
| 2’-F-2’-dG | ||
| 2’-F-2’-dU | ||
| Spacer | dSpacer | |
| Spacer C3 | ||
| Spacer C9 | ||
| Spacer C18 |
Single Labeling
Synbio Technologies provides multiple fluorescent groups at 3’, 5’ or designated sites to comply with our customers’ requests. This includes FAM, HEX, ROX, BHQ, Texas Red, etc.
| Dye/Quenchers | 3′- | 5′- | Designated Sites | Purification |
|---|---|---|---|---|
| FAM | √ | √ | √ | HPLC/PAGE |
| TET | √ | √ | √ | |
| JOE | √ | √ | √ | |
| HEX | √ | √ | √ | |
| SIMA | √ | √ | √ | |
| TAMRA | √ | √ | √ | |
| ROX | √ | √ | √ | |
| Cy5 | √ | √ | √ | |
| Cy3 | √ | √ | √ | |
| Quasar670 | √ | √ | ||
| Quasar570 | √ | √ | ||
| DyLight547 | √ | |||
| DyLight647 | √ | |||
| Texas Red | √ | |||
| BHQ-1 | √ | |||
| BHQ-2 | √ | |||
| BHQ-3 | √ | |||
| Eclipse | √ | |||
| DABCYL | √ |
Dual/Multiple Labeling
Synbio Technologies also offers dual/multiple labeling services including TAMRA, BHQ, DABYCL, ELCISPE, etc. The most common Dyes and Quenchers are listed below:
| Dyes | Quenchers | Purification |
|---|---|---|
| 5’6-FAM | 3’TAMRA | HPLC/PAGE |
| 3’BHQ-1 | ||
| 3’Eclipse | ||
| 3’DABCYL | ||
| 5’TET | 3’TAMRA | |
| 3’BHQ-2 | ||
| 3’DABCYL | ||
| 5’HEX | 3’TAMRA | |
| 3’BHQ-1 | ||
| 3’BHQ-2 | ||
| 3’DABCYL | ||
| 5’TAMRA | 3’Eclipse | |
| 3’BHQ-1 | ||
| 3’BHQ-2 | ||
| 3’DABCYL | ||
| 5’ROX | 3’Eclipse | |
| 3’BHQ-1 | ||
| 3’BHQ-2 | ||
| 3’DABCYL | ||
| 5’CY5 | 3’BHQ-1 | |
| 3’BHQ-2 | ||
| 3’BHQ-3 | ||
| 3’DABCYL | ||
| 5’CY3 | 3’BHQ-2 | |
| 5’JOE | 3’Eclipse | |
| 3’BHQ-1 | ||
| 3’BHQ-2 | ||
| 3’DABCYL | ||
| 3’TAMRA | ||
| 5’Texas Red | 3’BHQ-2 |
* The above is our suggested purification method. If you need other purification methods, please contact us for a quote.
Deliverables
Lyophilized dry DNA oligo powder, 1 tube (Transparent or dark) or 96-well and 384-well plates.
Certificate of Analysis (COA): including sequence information, OD, Tm, etc.
FAQs
How many types of modifier Oligos are there?
There are more than 300 types of modifier oligos.
Why are modified oligos less productive and more expensive than standard oligos?
The main reason is that the modified monomer is less stable, the coupling time is long, the efficiency is low, and the yield obtained at the end is naturally lower than the general oligos.
Modified oligos usually need to be purified by PAGE or HPLC and the purification process is difficult. Alongside this, you will need many more raw materials (on the order of 100x more.) Both of these factors cause modified oligos to be naturally more expensive.
Do modifier labels have an effect on OD measurements?
Some fluorescent groups absorb light at 260 nm, e.g. FAM, HEX, TAMRA, TET. Others may have absorption values at 260 nm, but the data is not published/verified and has therefore not been included in the calculation.
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