About Unstable GFP (destabilized GFP) :

The green fluorescent protein (GFP) is a widely used reporter, providing an easy detection in living cells. However, it is a very stable protein and accumulated in cells with long half-live, which limits its application that requires fast turnover responses in signal pathway assay and in knockdown / knockout detection. Therefore, the unstable GFP (uGFP) was created as the destabilized version reporter. The uGFP is best used for the time course induction and dose-response kinetics, and for the fast response to knockdown or knockout.

Lentivirus express the unstable GFP:

Gentarget developed the expression lentivirus for the destabilized GFP (uGFP) (click to see its sequence) fluorescent protein (475nm/510-525nm, Ex/Em), driven by an optional inducible CMV promoter (TetCMV) with different antibiotic selection marker. See core lentivector scheme below.

Unstable GFP lentivector map

Lentivirus are provided as in two formats:

Crude Lentivirus: 200ul /vial in DMEM containing 10 x Polybrene;
Concentrated lentivirus: 200ul PBS solution which is better for hard-to-transduced cell types or for serum-free culture.

see click Product Manual (.pdf).for details

Unstable GFP

Product name	Cat. No.
uGFP (TetCMV-Bsd) Lentivirus in PBS	LVP1109-PBS
uGFP (TetCMV-Neo) Lentivirus in PBS	LVP1110-PBS
uGFP (TetCMV-Puro) Lentivirus in PBS	LVP1108-PBS