About rtTA (reverse tetracycline Transcriptional Activator):

rtTA binds to the tetracycline operator element (TetO) in the presence of doxycycline (Dox), which turn on the expression. Any promoters embedded with TetO sequences can bind to the rtTA protein when the inducer (Dox or tetracycline) is present, activating transcription of the downstream gene (the so-called Tet-On inducible system).

rtTA-M2 (ABC65845.1) is a mutant of rtTA that has increased stability and reduced background expression in the absence of the tetracycline or Dox and improved inducibility in the presence of Tet / Dox.

rtTA Expression Lentivirus:

Gentarget provides the Lentivirus expressing the reverse tetraccycline transcription activator gene, rtTA-M2 under an enhanced EF1α promoter. It also contains a antibiotic selection (Neo, Puro, Bsd or a fluorescent-antibiotic fusion dual marker) under a Rsv promoter.

Please see the core map scheme below for details.

rtTA Expression vector scheme

See Product Manual (.pdf).

Reference:

PNAS, July 5, 2000, V97(14), 7963-7968
BMC Developmental Biology 2010, 10:17

rtTA Expression

Product name	Cat. No.
rtTA (Bsd) Lentiviral particles in PBS	LVP400-Bsd-PBS
rtTA (GFP-Bsd) Lentiviral particles in PBS	LVP400-GB-PBS
rtTA (GFP-Puro) Lentiviral particles in PBS	LVP400-GP-PBS
rtTA (Hygro) Lentiviral particles in PBS	LVP400-Hygro-PBS
rtTA (Neo) Lentiviral particles in PBS	LVP400-Neo-PBS
rtTA (Puro) Lentiviral particles in PBS	LVP400-Puro-PBS
rtTA (RFP-Bsd) Lentiviral particles in PBS	LVP400-RB-PBS
rtTA (RFP-Puro) Lentiviral particles in PBS	LVP400-RP-PBS
rtTA (Zeo) Lentiviral particles in PBS	LVP400-Zeo-PBS