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SARS-CoV-2 Neutralizing Antibody

제품 번호

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제품 특징

Rabbit monoclonal anti-SARS-CoV-2 spike RBD neutralizing antibody

This rabbit monoclonal antibody was raised against the RBD domain of the SARS-CoV-2 S1 protein (Arg319 to Phe541, sequence below). The antibody was validated through binding assays (ELISA) and neutralization assays for its ability to neutralize SARS-CoV-2 pseudoviruses such as Ha-CoV-2 and Ha-CoV-2 variants (e.g. delta and omicron). The antibody was expressed in mammalian cells and purified as a recombinant protein. Antibody 27VBI has an extremely high affinity to RBD, and is be able to detect as low as 0.5 pg/ml RBD protein in human serum. For more information, please contact us by email: info@virongy.com

RBD antigen protein sequence:

rvqptesivrfpnitnlcpfgevfnatrfasvyawnrkrisncvadysvlynsasfstfkcygvsptklndlcftnvyadsfvirgdevrqiapgqtgkiadynyklpddft

gcviawnsnnldskvggnynylyrlfrksnlkpferdisteiyqagstpcngvegfncyfplqsygfqptngvgyqpyrvvvlsfellhapatvcgpkkstnlvknkcvnf

Application

• ELISA – Clone 27VBI has been validated for ELISA assay to quantify of SARS-CoV-2 spike protein.
• Neutralizing antibody assay – Clone 27VBI has been validated in neutralizing antibody assays for neutralizing SARS-CoV-2 pseudoviruses such as Ha-CoV-2 and Ha-CoV-2 variants.
• Western blot – not tested
• Fluorescent imaging – not tested

Left, 27VBI was used in an ELISA assay to quantify SARS-CoV-2 spike RBD protein. Right, RBD protein was mixed with human serum (0.5 pg/ml) and then detected by 27VBI in an ELISA assay.

Left, 27VBI was used in Ha-CoV-2 pseudovirus neutralizing antibody assay. Right, 27VBI was used in Ha-CoV-2 (Omicron) variant pseudovirus neutralizing antibody assay.

Application

• ELISA – Clone 27VBI has been validated for ELISA assay to quantify of SARS-CoV-2 spike protein.
• Neutralizing antibody assay – Clone 27VBI has been validated in neutralizing antibody assays for neutralizing SARS-CoV-2 pseudoviruses such as Ha-CoV-2 and Ha-CoV-2 variants.
• Western blot – not tested
• Fluorescent imaging – not tested

Left, 27VBI was used in an ELISA assay to quantify SARS-CoV-2 spike RBD protein. Right, RBD protein was mixed with human serum (0.5 pg/ml) and then detected by 27VBI in an ELISA assay.

Left, 27VBI was used in Ha-CoV-2 pseudovirus neutralizing antibody assay. Right, 27VBI was used in Ha-CoV-2 (Omicron) variant pseudovirus neutralizing antibody assay.

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