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AntibodySystem

[Antibodysystem] Anti-Adalimumab ELISA Kit

Cat-No. KAB94401

AntibodySystem는 Primary Antibody Biosimilar - Research Grade

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제품 설명

Anti-Adalimumab ELISA Kit

제품 번호

KAB94401

제품 특징

Catalog No.

KAB94401

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Adalimumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Adalimumab will be captured by immobilized Adalimumab. After washing away any unbound substances, a biotin-labeled Adalimumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Adalimumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-Adalimumab concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

1.56 - 100 ng/mL

Sensitivity

0.81 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	46.5	11.0	2.8	50.2	12.4	3.0
Standard deviation	1.8	0.9	0.3	7.2	0.7	0.3
CV (%)	3.9	8.4	10.1	14.3	5.4	10.9

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

ABP 501,D2E7, Adalimumab,CAS: 331731-18-1

Data Image

Experiment Example
CALCULATION OF RESULTS
Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Anti-Adalimumab concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.