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AntibodySystem

[Antibodysystem] Anti-Bevacizumab ELISA Kit

Cat-No. KAD12601

AntibodySystem는 Primary Antibody Biosimilar - Research Grade

제품을 전문적으로 생산하는 20년의 경험이 있는 High Quality 제조사입니다.

제품 설명

Anti-Bevacizumab ELISA Kit

제품 번호

KAD12601

제품 특징

Catalog No.

KAD12601

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Bevacizumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Bevacizumab will be captured by immobilized Bevacizumab. After washing away any unbound substances, a biotin-labeled Bevacizumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Bevacizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-Bevacizumab concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

9.38 - 600 ng/mL

Sensitivity

5.78 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	290.2	71.3	15.2	294.6	72.8	15.5
Standard deviation	8.9	2.4	1.3	6.8	2.3	1.7
CV (%)	3.1	3.4	8.7	2.3	3.2	11.1

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

12-IgG1,F(ab)-12 IgG1,Fab-12 IgG1,rhuMAb-VEGF, ABP 215,CAS: 216974-75-3

Data Image

Experiment Example
CALCULATION OF RESULTS
Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Anti-Bevacizumab concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.