모아바이오는 미국 Biological 생산업체인 Neuromics의 공식 한국 대리점 입니다. 

제품 설명

Immortalized Human Lung Squamous Cell Carcinoma CAFs

제품 번호

CAF117S-IM

제품 특징

Product Details

Catalog Number: CAF117S-IM

Applications: Cell Assays

Type: Human Primary CAFs

Storage: Keep frozen in liquid nitrogen until plating

Shipping: Frozen (Dry Ice/Liquid Nitrogen)

Format A: Frozen

Species Reactivity: Human

Downloads: Datasheet (pdf)

Product Sizes: 1,000,000 Cells

Carcinoma associated fibroblasts (CAFs) have recently been implicated in important aspects of epithelial solid tumor biology such as neoplastic progression, tumor growth, angiogenesis, and metastasis. Human Lung Squamous Cell Carcinoma CAFs are isolated from human lung tumor tissue. Cells are grown in T75 tissue culture flasks.

Then, cells are infected with hTert-lentiviral particles under specially formulated media. A Puromycin resistant gene was expressed under RSV promoter, which allows selecting the transduced cell via Puromycin antibiotic hTERT. Normal CAFs can be passaged 3-5 times, while immortalized CAFs can be passaged >10 times.

CAFs can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.

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