Boster Bio는 생명과학 연구를 위한 항체, ELISA 키트, 단백질 및 관련 제품을 제공하는 미국의 생명과학 기업입니다.
Rat CXCL4/PF4 ELISA Kit PicoKine®
EK1651
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat CXCL4/PF4 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Pf4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Rat CXCL4/PF4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1651)
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: V30-S105
<15 pg/ml
156 pg/ml - 10,000 pg/ml
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1651 for more details
There is no detectable cross-reactivity with other relevant proteins.
EK1651 is reactive to PF4 in Rat samples
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1651 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1651-CAP | Anti-Rat PF4 Pre-coated 96-well strip microplate | 1 |
| EK1651-ST | Rat PF4 Standard | 2 vials, 10 ng/tube |
| EK1651-DA | Rat PF4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.062 | 0.116 | 0.152 | 0.237 | 0.531 | 0.922 | 1.368 | 1.983 |
Click image to see more details
Rat CXCL4 PicoKine ELISA Kit standard curve
According to our internal validation assays using this ELISA kit, to detect CXCL4/PF4, Dilution ratio of 1:100000, concentration in serum and plasma is around 100 ug/ml..
We measured random samples of Rat CXCL4/PF4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 205 | 1658 | 5065 | 199 | 1676 | 4964 |
| Standard deviation | 8.81 | 72.95 | 379.87 | 9.35 | 87.15 | 416.97 |
| CV (%) | 4.3% | 4.4% | 7.5% | 4.7% | 5.2% | 8.4% |
We ensure reproducibility by testing three samples with differing concentrations of CXCL4/PF4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 205 | 211 | 226 | 204 | 211 | 8.78 | 4.1% |
| Sample 2 | 1658 | 1447 | 1691 | 1522 | 1579 | 99.31 | 6.2% |
| Sample 3 | 5065 | 4548 | 5213 | 4497 | 4830 | 313.17 | 6.4% |
PF4
Platelet factor 4
intercrine alpha (chemokine CxC) family
chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A PF4 CXCL4, PF-4, SCYB4 platelet factor 4 platelet factor 4|C-X-C motif chemokine 4|chemokine (C-X-C motif) ligand 4|iroplact|oncostatin-A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".In this infographic you will see the following information for PF4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
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