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Boster Bio

[Boster Bio] HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

Cat-No. BA1054

Boster Bio는 생명과학 연구를 위한 항체, ELISA 키트, 단백질 및 관련 제품을 제공하는 미국의 생명과학 기업입니다.

Product Info Summary

SKU:	BA1054
Size:	0.5ml
Reactive Species:	Rabbit
Host:	Goat
Application:	ELISA, WB

Product Overview

Product Name	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Synonyms	HRP-conjugated goat anti-rabbit IgG
Description	Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
Reagent Type	Secondary antibody, reporter enzyme labeled
Label	HRP (Horseradish Peroxidase)
Host	Goat
Target Species	Rabbit
Antibody Class	IgG
Clonality	Polyclonal
Immunogen	Rabbit IgG (whole molecular)
Purification	The antibody was purified from antisera by immunoaffinity chromatography.
Specificity	This HRP conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.
Form Supplied	Concentrated, Liquid
Formulation	0.5 mg of HRP conjugated specific antibody 0.01 M PBS (PH 7.4) 50% glycerol
Pack Size	0.5 ml
Concentration	1mg/ml
Application	ELISA, WB *Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage	At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
Precautions	FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type	SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type	Immunoassay
Assay Purpose	Protein detection/quantification
Technique	Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed	WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents	Incompatible with sodium azide and metals incompatible with high phosphate concentrations

Main Advantages

Specific	High signal-to-noise ratio
Sensitive	Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification	Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast	Generates strong signals in a relatively short time span
Quantifieable	Allows quantification of detected signal
Easy to Use	Supplied in a workable liquid format
Flexible	HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient	HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.