Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.
제품 설명
50 mM MgCl2
제품 번호
BR1900101
제품 특징
Description
biotechrabbit™ MgCl2 is ideally suited for optimization of PCR conditions. For convenience and flexibility, it is provided as 50 mM solution.
Other Information
Component | Composition |
50 mM MgCl2 | PCR Grade 50 mM MgCl2 in water |
STORAGE | -20°C (until expiry date – see product label) |
Human genomic DNA was amplified using Taq DNA Polymerase and specific primers to produce a distinct band of 750 bp.
Protocols
Many applications use the standard concentration of 1.5 mM MgCl2. However, reactions with increased amounts of template (e.g., genomic DNA), primer and nucleotides might require higher MgCl2 concentrations (2–3 mM). The 50 mM MgCl2 solution can be used to adjust the MgCl2 concentration according to the table below:
Final concentration of MgCl2 in a 50 µl reaction, mM | 1.5 | 1.75 | 2.0 | 2.5 | 3.0 |
Volume of 50 mM MgCl2 solution to add, µl | 1.5 | 1.75 | 2.0 | 2.5 | 3.0 |
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