YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

2× Hieff Canace™ AdvanceFast High-Fidelity PCR Master Mix (With Dye) 

제품 번호

10164ES01 / size: 250μl

10164ES03  / size: 1ml

10164ES08 / size: 5x1ml 

제품 설명

Product description

2× Hieff Canace™ AdvanceFast PCR Master Mix (With Dye) is a ready-to-use 2× pre-mixed solution containing Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase, dNTPs, and an optimized buffer system, which contains pre-added electrophoresis indicators. The pre-mix contains pre-added electrophoresis indicator, PCR products can be directly electrophoresed, the amplification products are flat ends. 2× Hieff Canace™ AdvanceFast PCR Master Mix (With Dye) has the advantages of quick and easy, high sensitivity, high specificity, good stability, etc., the reaction system can be added with only the primers and templates. In addition, the product also contains a specific protective agent, so that the premix can still maintain stable activity after repeated freezing and thawing.

Ultra-high fidelity: Low mismatch rate in mutation-prone high GC gene;
Fast speed: The speed is as fast as 5 sec/kb;
Wide applicability: It can be used to amplify genes with 20-80% GC content, and tolerate 20% blood and mouse lysates;
Good stability: Stored at 37°C for 7 days without affecting performance;
Easy use: Just adding primers and templates for amplification, with blue loading dye for direct electrophoresis.

Specifications

Figures

Figure: Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high fidelity compared to other brands' products (A). The assay was done by examining the mutation frequency of PCR products of 6 GC-rich (about 80%) segments amplified from the genomes of different species. The PCR products amplified by Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase were cloned into vectors, and 100 selected single clones for each sequence were subjected to Sanger sequencing.

Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high amplification speed (B), high GC amplification compatibility (C), and blood tolerance (D). Results from customers' PCR and sequencing show that Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has good performance in PCR yields and specificity (E-H).

Storage

This product should be stored at -25~-15℃ for 1 years.

Instructions 

1. Recommended PCR reaction systems.

Table 1 PCR reaction system

*In 1× premixes containing 2 mM Mg2+ and 200 μM dNTPs.

**Recommended range 10-200 ng, cDNA sample upload volume range not more than 1/10 of the reaction system, recommended 1-2.5 μL.

***The final primer concentration in the PCR reaction system ranges from 0.2-1 μM, and 0.4 μM is recommended.

2. Reaction program.

Table 2 PCR reaction program

*Recommended temperature: 60°C, a temperature gradient can be set up to find the optimal temperature for primer annealing. The recommended annealing time is set to 5 sec and can be adjusted from 5-30 sec. Too long annealing time may result in diffuse amplification products on the gel.

**Extension time: Recommended 5 sec/kb, can also be extended to 10 sec/kb as needed.

Notes

1. This product is for research use only.

2. Please operate with lab coats and disposable gloves，for your safety.

YEASEN의 모든 제품을 만나 보세요!

Biopharma

mRNA enzyme

Nucleotides

Viral Vector Manufacturing

Quality Analysis Kit

NGS Library Preparation

Adapter

DNA Library Preparation

RNA Library Preparation

NGS Module

NGS enzyme

Magnetic Bead

Library Quantitation

Molecular Diagnostics

Glycerol-free Enzyme

RT-qPCR Enzyme

RT-LAMP Enzyme

dNTPs

IVD Kit

Life Science Reagents

Hot Products

Molecular Biology

Cell Culture & Analysis

Protein Research

Animal Model

Others

YEASEN - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"