본문 바로가기 주메뉴 바로가기

제품소개

제품상세페이지

YEASEN

[YEASEN] Hifair™ V Multiplex One Step RT-qPCR Probe Kit(UDG Plus)

Cat-No. 13650ES
13650ES50 / size: 50T
13650ES60 / size: 100T
13650ES80 / size: 1000T

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.




제품 설명


Hifair™ V Multiplex One Step RT-qPCR Probe Kit(UDG Plus)




제품 번호


13650ES50 / size: 50T

13650ES60 / size: 100T

13650ES80 / size: 1000T




제품 설명


Product description

Hifair™ V Multiplex One Step RT-qPCR Probe Kit (UDG Plus) is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.

In this kit, the first strand cDNA was efficiently synthesized by heat-resistant Hifair V Reverse Transcriptase and quantitatively amplified by UNICON HotStart Taq DNA Polymerase. The kit mainly contains optimized MP buffer, enzymes mix, etc. The buffer solution already contains Mg2+ and dNTP. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction. The dUTP/UDG system was added to effectively prevent the risk of aerosol contamination.


Specifications

PCR method

One step RT-qPCR

Type of sample

RNA

Application equipment

Equipment with RoxABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus

Equipment with Low RoxABI 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex

Stratagene MX3000P™, MX3005P™, MX4000P™

Equipment without Rox

Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon® 2, Chromo4™

Eppendorf Mastercycler® ep realplex, realplex 2 s; Qiagen Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000

Roche Applied Science LightCycler® 480, LightCycler® 2.0, Lightcycler® 96

Thermo Scientific PikoReal Cycler; Cepheid SmartCycler®Illumina Eco qPCR


Components

Components No.

Name

13650ES50  (50 T)  

13650ES60 (100 T)

13650ES80 (1,000 T)

13650ES92 (10,000 T)

13650-A

2×Hifair V MP Buffer

750 μL

1.5 mL

15 mL

150 mL

13650-B

Hifair V Enzyme Mix

60 μL

120 μL

1.2 mL

12 mL

Note1) 2×Hifair V MP Buffer is the abbreviation of Hifair V Multiplex One Step RT-qPCR Probe Bufferwhich includes dNTPs, dUTP, Mg2+, stabilizers, enhancers, and more.

2) Hifair V Enzyme Mix mainly contains heat-resistant Hifair V reverse transcriptase, UNICON HotStart Taq DNA polymeras and UDGase.


Storage

The product should be stored at -25°C~-15°C for 1 year.


Instructions

  1. Reaction Composition

Components

Volume (μL)

Final Concentration

2×Hifair V MP Buffer

15

Hifair V Enzyme Mix

1.2

-

Primer / Probe Mix (2.5 μM)

3

0.25 μM

Template RNA

1-10

-

RNase Free H2O

to 30

-

NoteBe sure to mix well before use, avoid excessive bubbles caused by violent vibration.

a) Primer concentration: Primer mix including multiplex primer, depending on the situation optimal primer concentration may be between 0.1 and 1.0 μM.

b) Probe concentration: Probe mix including multiplex probe labeling difference fluorescent group, depending on the situation optimal probe concentration may be between 0.05 and 0.5 μM.

c) Template dilution: qPCR is highly sensitive and it is recommended to dilute the template. The control Ct value is suitable between 20 and 35.

d) System preparation: un head with filter element. Avoid cross contamination and aerosol contamination.

  1. Optimized Cycling Protocol

 

Reaction stage

Temperature

Time

Cycle

1

Reverse transcription

50°Ca

10 min

1

2

Initial denaturation

95°C

5 min

1

3

Amplification reaction

95°C

15 sec

45 cycles

60°Cb

30 secc

Notea) Reverse transcription: The temperature can select 42°C or 50°C for 10-15 minutes.

b) Amplification reaction: The temperature is adjusted according to the Tm value of the designed primers.

c) Fluorescence signal acquisition: Please set the experimental procedure according to the requirements of the instrument manual.




YEASEN의 모든 제품을 만나 보세요!


Biopharma

mRNA enzyme

Nucleotides

Viral Vector Manufacturing

Quality Analysis Kit


NGS Library Preparation

Adapter

DNA Library Preparation

RNA Library Preparation

NGS Module

NGS enzyme

Magnetic Bead

Library Quantitation


Molecular Diagnostics

Glycerol-free Enzyme

RT-qPCR Enzyme

RT-LAMP Enzyme

dNTPs

IVD Kit


Life Science Reagents

Hot Products

Molecular Biology

Cell Culture & Analysis

Protein Research

Animal Model

Others


YEASEN - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"