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제품 설명

Rat ACH(Acetylcholine) ELISA Kit

제품 번호

EK248979

제품 특징

시약 준비 과정

• Ensure all kit components and samples are brought to room temperature (18-25°C) before use. Thoroughly dissolve and mix all components before utilizing the kit./li>
• If the entire kit will not be used at once, only remove the necessary strips and reagents for the current experiment. Store the remaining strips and reagents as directed.
• Dilute the 25× Wash Buffer into 1× Wash Buffer with double distilled water.

Standard Working Solution

First, centrifuge the Standard at 1000 x g for 1 minute. Then, reconstitute the Standard by adding 1.0 ml of Standard Diluent Buffer. Allow the mixture to sit for approximately 10 minutes at room temperature, and gently shake it (avoiding foaming).

Prepare 7 tubes with 0.5 ml of Standard Diluent Buffer in each and create a series of dilutions using the Diluted Standard. Remember to thoroughly mix each tube before transferring the solution to the next tube by pipetting the solution up and down several times. Set up 7 points of Diluted Standard, decreasing the concentration each time, and use a Blank with no standard for comparison. It's important to use a new Standard Solution for each experiment to ensure accurate results. When diluting the Standard from high concentration to low concentration, replace the pipette tip for each dilution. Additionally, make sure not to transfer solution into the Blank tube from the previous one.

• Before using the stock Biotinylated-Conjugate and Streptavidin-HRP, give them a quick spin. Then, dilute them to the working concentration, for example, 10 μL of Streptavidin-HRP with 990 μL of HRP Diluent.
• When using the TMB Substrate Solution, be sure to aspirate the required amount with sterilized tips and avoid returning any leftover solution to the vial.

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  결과 값 계산

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• Take the standard concentration as the horizontal, the OD value for the vertical, draw the standard curve on graph paper, find out the corresponding concentration according to the sample OD value by the sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard concentration and the OD value, with the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor, the result is the sample actual concentration. 

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