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Biotechrabbit

[Biotechrabbit] GenUP™ BS Virus RNA Kit

Cat-No. BR0702303


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


GenUP™ BS Virus RNA Kit



제품 번호


BR0702303


제품 특징


Features

  • Fast and simple procedure
  • High yields of pure RNA
  • No DNase treatment, no toxic β-mercaptoethanol

Applications

  • Virus RNA isolation from swabs



Description


biotechrabbit™ GenUP BS Virus RNA Kit has been specially developed for quick and easy isolation of viral RNA from swabs, including nasopharyngeal and oropharyngeal swabs. The unique binding membrane of our high-capacity Mini Filters guaranties high yields. A high concentration of purified RNA can be achieved with flexible elution volumes. The kit includes carrier RNA.

After few initial steps, the viral RNA is bound to a Mini Filter, washed, and then eluted in a separate tube. The purified RNA is ready to be used in all demanding molecular biology applications, including qRT-PCR.



Other Information


STARTING MATERIAL

Swabs samples

EXTRACTION TIME

Approximately 25 min

TYPICAL YIELD

Yield is dependent on sample quality and viral load



Protocols

STEPS

  • Extract swab and lyse with Proteinase K.
  • Centrifuge.
  • Add isopropanol, mix and transfer to a Mini Filter.
  • Centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Wash 2 times with 80% ethanol and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Add pre-heated Water, RNase-free, incubate and centrifuge.
  • RNA is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).



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