Minerva Biolabs는 세포배양 및 바이오의약품에서의 mycoplasma, bacteria, virus를 검출/오염방지/제거 등 컨트롤할 수 있는 

고품질의 다양한 키트와 제품을 제공하는 독일 소재의 제조사입니다. 

[NEW!] 신제품

제품 설명

MiQuant® CAR-T Cell dPCR Lenti

제품 번호

58-0701 (24 Reactions)

58-0702 (4x24 Reactions)

제품 특징

The MiQuant® CAR-T Cell dPCR Lenti kit is intended for the determination of vector copy numbers (VCN) in transduced cells to determine the average copies of integrated lentiviral vectors per cell genome after ex vivo transduction of human or other mammalian cells. This kit works for all lentivirus transduction systems.

Type of PCR

Digital PCR (dPCR)

Recommended Use

MiQuant® CAR-T Cell dPCR Lenti can be used for the characterization of cellular products and for in-process control as well as final QC release testing in cell therapy production processes.
Besides these applications the MiQuant® CAR-T Cell dPCR Lenti kit can also be used for determining VCN in cell culture samples in research and development and is applicable for human or other mammalian cells (such as mice and rats).

Kit Components

• MiQuant® Lenti Mix (lyophilized)
• Positive Control DNA (lyophilized)
• Rehydration Buffer
• dPCR grade Water

Package Size

Cat. No. 58-0701: 24 reactions

Cat. No. 58-0702: 4 x 24 reactions

Result Evaluation

Absolute Quantification

Required Consumables

• QIAcuity® Nanoplate 26k 24-well
• MiQuant® SP Beads (sample preparation kit, Cat. No. 56-4010/-4100)
• 1.5 ml reaction tubes, DNA- and RNA-free

Optional:

• VCN Standard (Cat. No.58-0801)
• Restriction enzymes (EcoRI-HF and NruI-HF; 20 U/μl)
• PCR Clean™ (Cat. No. 15-2025 and 15-2001)

Required Lab Device

• QIAcuity® digital PCR device
• Microcentrifuge for 1.5 ml PCR tubes
• Vortex mixer
• Pipettes with corresponding filter tips

Shelf Life and Storage

Shipment at ambient temperature. The expiry date of the unopened package is marked on the package label. The kit components must be stored at +2 to +8°C until use. The rehydrated components must be stored at ≤ -18°C for a maximum of one month.

Calculate the Vector Copy Number with our tool.
Data Analysis Application

Minerva Biolabs의 모든 제품을 만나보세요!

Cell Culture Contamination Control

DNA / RNA Extraction

PCR Reagents & Enzymes

Pharma / ATMP

Decontamination Reagents for PCR Labs

Water Contamination Testing

Proficiency Testing Program 2024

Contract Manufacturing

Minerva Biolabs - Official Distributor in South Korea "Morebio" 한국 독점 대리점 "모아바이오"