AntibodySystem는 Primary Antibody Biosimilar - Research Grade 제품을 전문적으로 생산하는 20년의 경험이 있는 High Quality 제조사입니다. 

제품 설명

Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (A35R)

제품 번호

KAV13101

제품 특징

PRINCIPLE OF THE ASSAY. This assay employs the quantitative competitive enzyme immunoassay technique. A recombinant Monkeypox virus A35R antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox Virus IgG (A35R) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox Virus IgG (A35R). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox Virus IgG (A35R) bound in the initial step. The color development is stopped and the intensity of the color is measured.

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  Experiment Example
  CALCULATION OF RESULTS
  
  Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Human Anti-Monkeypox Virus IgG (A35R) concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
  
  If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

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