The process of creating a recombinant monoclonal antibody against TPSB2 began with the immunization of a rabbit using a synthesized peptide derived from human TPSB2 protein. B cells were isolated from the immunized rabbit, and RNA was extracted from these B cells. The RNA was reverse-transcribed into cDNA, which served as a template for extending TPSB2 antibody genes using degenerate primers. The extended TPSB2 antibody genes were then incorporated into a plasmid vector and introduced into host cells for expression. Subsequent purification of the TPSB2 recombinant monoclonal antibody from t...Read more