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제품 설명 

Anti-MPXV A35R Human IgG ELISA Kit

제품 번호

KAV13102

제품 특징

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV A35R antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human Anti-MPXV A35R IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-MPXV A35R IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

A35R

Applications

Used for the quantitative determination of Human Anti-MPXV A35R IgG concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

0.625 - 20 ng/mL

Sensitivity

0.62 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.