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ELISA Kits, Antibody, Protein, Reagents 등 생명과학 연구에 필요한 다양한 제품을 제공합니다.
제품 설명
Mouse Anti-MPV IgM(Anti-Monkeypox Virus IgM) ELISA Kit
제품 번호
EH2128
제품 설명
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
| STD.(ng/ml) | OD-1 | OD-2 | Average |
|---|---|---|---|
| 0 | 0.103 | 0.107 | 0.105 |
| 1.562 | 0.17 | 0.174 | 0.172 |
| 3.125 | 0.227 | 0.233 | 0.23 |
| 6.25 | 0.37 | 0.38 | 0.375 |
| 12.5 | 0.587 | 0.605 | 0.596 |
| 25 | 0.971 | 0.999 | 0.985 |
| 50 | 1.694 | 1.744 | 1.719 |
| 100 | 2.232 | 2.296 | 2.264 |
Add a certain amount of Anti-MPV IgM into the sample. Calculate the recovery by comparing the measured value with the expected amount of Anti-MPV IgM in the sample.
| Sample Type | Recovery Range(%) | Average(%) |
|---|---|---|
| serum(n=10) | 96-102 | 100 |
| EDTA plasma(n=10) | 86-103 | 97 |
| Heparin plasma(n=10) | 85-102 | 96 |
Dilute the sample with a certain amount of Anti-MPV IgM at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=10) | 88-102% | 85-92% | 91-100% |
| EDTA plasma(n=10) | 85-104% | 89-100% | 85-97% |
| Heparin plasma(n=10) | 86-95% | 87-100% | 88-99% |
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item | Intra-assay Precision | Inter-assay Precision | ||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/ml) | 3.07 | 12.18 | 47.85 | 3.11 | 11.87 | 46.35 |
| Standard deviation | 0.14 | 0.63 | 2.19 | 0.17 | 0.7 | 1.97 |
| CV(%) | 4.58 | 5.16 | 4.58 | 5.32 | 5.86 | 4.26 |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months |
| Average(%) | 80 | 95-100 |
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