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Elabscience

[Elabscience] Elab Fluor® 647-11-dUTP

Cat-No. E-CK-A124D

Elabscience는 ELISA Kits / Antidbodies / Protein을 전문적으로 생산하는 미국의 생명과학 기업입니다.

제품 설명

Elab Fluor® 647-11-dUTP

제품 번호

E-CK-A124D

제품 특징

Detection principle

Chromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3'-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescen labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of terminal deoxyribonucleotide transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometer or fluorescence microscope.

General Information

Application	DNA Fragmentation
Detection method	Fluorometric Method
Sample type	Paraffin section, frozen section, cell slide
Detection instrument	Fluorescence Microscope
Dye Type	Elab Fluor^® 647
Ex/Em (nm)	650/665
Filter set	Cy5
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A321	One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor^® 488)
E-CK-A211	Annexin V-FITC/PI Apoptosis Kit
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Data Image

Hela cells was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample.(Red)

Paraffin embedded mouse heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red).