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Elabscience

[Elabscience] Calcein AM Solution (100 μM)

Cat-No. E-CK-A164

Elabscience는 ELISA Kits / Antidbodies / Protein을 전문적으로 생산하는 미국의 생명과학 기업입니다.

제품 설명

Calcein AM Solution (100 μM)

제품 번호

E-CK-A164

제품 특징

Detection principle

Elabscience® Calcein AM Solution (100 μM) can be used to distinguish living cells in mammals with esterase activity. Calcein AM is the addition of acetyl methoxy methyl ester (AM) group to traditional Calcein, which increases hydrophobicity and can easily penetrate the living cell membrane and enter the cell. Calcein AM itself has no fluorescence. After entering the cell, it is hydrolyzed by endogenous esterase in the cell to produce Calcein, a polar molecule with strong negative charge and cannot be retained in the cell through the cell membrane, while Calcein can emit strong green fluorescence ( Ex/Em = 494nm/517nm ). Due to the lack of esterase, dead cells cannot or rarely produce Calcein, so only living cells are stained with strong green fluorescence, and dead cells cannot be stained or stained very weakly. Compared with other live cell staining probes, Calcein AM is less toxic and does not affect cell differentiation and proliferation.

General Information

Application	Viability/Cytotoxicity
Detection method	Fluorometric Method
Sample type	Cell samples
Assay time	30min
Detection instrument	Flow Cytometry, Fluorescence Microscope
Dye Type	Calcein
Ex/Em (nm)	494/517
Channel set	FITC
Filter set	FITC
Other reagents required	PBS (pH7.2~7.6)(E-IR-R187).
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A153	Calcein AM Assay Buffer
E-CK-A165	Propidium Iodide (PI) Solution(750 μM)
E-CK-A354	Calcein AM/PI Double Staining Kit

Data Image

4T1 cells and Hela cells were treated with 5 % DMSO, 5μM Camptothecin, 10μM Camptothecin for 4h, then stained with Calcein-AM / PI Double Staining Kit and photographed.