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Elabscience

[Elabscience] Propidium Iodide (PI) Solution(750 μM)

Cat-No. E-CK-A165



Elabscience는 ELISA Kits / Antidbodies / Protein을 전문적으로 생산하는 미국의 생명과학 기업입니다.




제품 설명

 

Propidium Iodide (PI) Solution(750 μM)




제품 번호


E-CK-A165




제품 특징


Detection principle

PI is a kind of nucleic acid dye. Due to the loss of cell membrane selective permeability of dead cells, Propidium Iodide (PI) can enter the cell and specifically bind with double-stranded DNA and produce red fluorescence (Ex/Em=535nm/617nm), thus marking dead cells.

General Information

ApplicationViability/Cytotoxicity
Detection methodFluorometric Method
Sample typeCell samples
Assay time30min
Detection instrumentFlow Cytometry, Fluorescence Microscope
Dye TypePI
Ex/Em (nm)535/617
Channel setPE, PerCP/Cy5.5
Filter setTRITC
Other reagents requiredPBS (pH7.2~7.6)(E-IR-R187).
Storage-20°C, shading light
ShippingIce bag
Expiration date12 months




Data Image


4T1 cells and Hela cells were treated with 5 % DMSO, 5μM Camptothecin, 10μM Camptothecin for 4h, then stained with Calcein-AM / PI Double Staining Kit and photographed.


Molt-4 cells were treated with 5 % DMSO and 10 % DMSO for 4h, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.


Jurkat cells were placed at 4 °C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.




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