Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

1. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195

   Mahuang Xixin Fuzi decoction ameliorates apoptosis via the mitochondrial-mediated signaling pathway in MCM cells

   DOI: 10.1016/j.jep.2022.115538
   PMID: 35843410

2. Experimental and Therapeutic Medicine (2019) IF: 1.448

   Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B

   DOI: 10.3892/etm.2019.8192
   	Sample: Cell Sample

3. Experimental and Therapeutic Medicine (2019) IF: 1.448

   Downregulation of miR‑552 in hepatocellular carcinoma inhibits cell migration and invasion, and promotes cell apoptosis via RUNX3

   DOI: 10.3892/etm.2019.8061
   	Sample: Cell Sample

4. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943

   Antileishmanial activity of cordiaquinone E towards Leishmania (Leishmania) amazonensis

   DOI: 10.1016/j.intimp.2020.107124
   PMID: 33168414	Sample: Cell Sample

5. CELLULAR SIGNALLING (2022) IF: 4.85

   miR-24-3p down-regulates the expression of the apoptotic factors FasL and BIM in human natural killer cells

   DOI: 10.1016/j.cellsig.2022.110415
   PMID: 35870695

6. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932

   Curzerene antileishmania activity: Effects on Leishmania amazonensis and possible action mechanisms

   DOI: 10.1016/j.intimp.2021.108130
   PMID: 34500286

7. Cancer Cell International (2022) IF: 5.722

   A synthesized olean-28,13β-lactam targets YTHDF1-GLS1 axis to induce ROS-dependent metabolic crisis and cell death in pancreatic adenocarcinoma

   DOI: 10.1186/s12935-022-02562-6
   PMID: 35366902

8. Frontiers in Oncology (2021) IF: 6.244

   Discovering and Characterizing of Survivin Dominant Negative Mutants With Stronger Pro-apoptotic Activity on Cancer Cells and CSCs

   DOI: 10.3389/fonc.2021.635233

9. Acta Biomaterialia (2022) IF: 8.947

   Preparation of epigallocatechin gallate decorated Au-Ag nano-heterostructures as NIR-sensitive nano-enzymes for the treatment of osteoarthritis through mitochondrial repair and cartilage protection

   DOI: 10.1016/j.actbio.2022.03.038
   PMID: 35358735

10. ACS Applied Materials & Interfaces (2021) IF: 9.229

    Fully Integrated Self-Powered Electrical Stimulation Cell Culture Dish for Noncontact High-Efficiency Plasmid Transfection

    DOI: 10.1021/acsami.1c16748
    PMID: 34757708	Sample: A549 Cells,Hepg2 Cells

11. ACS Applied Materials & Interfaces (2022) IF: 10.383

    Ultrasound-Mediated Antitumor Therapy via Targeted Acoustic Release Carrier of Carbon Monoxide (TARC-CO)
    

    DOI: 10.1021/acsami.2c16821