(주)모아바이오

YEASEN

[YEASEN] SuperSignal MaxiSignal Maximum Sensitivity Substrate

Cat-No. 36224ES

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

SuperSignal MaxiSignal Maximum Sensitivity Substrate

제품 번호

36224ES10 - 10ml

36224ES60 - 100ml

36224ES70 - 200ml

36224ES76 - 500ml

제품 설명

Description

SuperSignal MaxiSignal Maximum Sensitivity Substrate is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle is that proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film.
This kit has a unique luminescent/enhanced substrate system, While reducing the exposure background, the introduction of a new oxidant greatly improves the stability of the kit, which can achieve low fick-grade western blotting detection. In addition to X-rays, can also be detected by the automatic imaging system.

Features

High sensitivity and high signal-to-noise ratio, it can detect low fick-grade antigens.
Long duration of luminescence, fluorescence can make X-ray film sensitive for more than 8 hours, especially suitable for detecting trace protein or nucleic acid.
Higher dilution ratio antibodies can be used to greatly save antibodies.
Dilution ratio of primary antibody (liquid storage concentration 1mg/mL) : 1:1000-1:100000.
Dilution ratio of secondary antibody (liquid storage concentration 1mg/mL): 1:100000-1:500000.

Applications

Chemiluminescence ELISA
Western Blot
Dot Blot-DNA/RNA
Southern blot-DNA
Northern blot-RNA

Specifications

Recommended Antibody Concentrations	primary antibody (liquid storage concentration 1mg/mL): 1:1000-1:100000 secondary antibody (liquid storage concentration 1mg/mL): 1:100000-1:500000
Signal Duration	< 8 h
Substrate Properties	Chemical Substrate
Substrate Type	HRP (Horseradish Peroxidase) Substrate
Sensitivity	low fick-grade antigens

Components

Components No.	Name	36224ES10(10 mL)	36224ES60(100 mL)	36224ES70(200 mL)	36224ES76(500 mL)
36224-A	MaxiSignal ECL - Reagent A	5 mL	50 mL	100 mL	250 mL
36224-B	MaxiSignal ECL - Reagent B	5 mL	50 mL	100 mL	250 mL