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YEASEN

[YEASEN] Anti-DYKDDDDK (Flag) Affinity Gel

Cat-No. 20585ES
20585ES01 - 100 μL
20585ES03 - 1 mL
20585ES08 - 5 mL
20585ES25 - 25 mL
20585ES60 - 100 mL

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.




제품 설명


Anti-DYKDDDDK (Flag) Affinity Gel




제품 번호


20585ES01 - 100 μL

20585ES03 - 1 mL

20585ES08 - 5 mL

20585ES25 - 25 mL

20585ES60 - 100 mL




제품 설명


Description

Anti-DYKDDDDK (Flag) Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with Sepharose 4B gel. This product has a high Flag label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Flag labels.
Anti-DYKDDDDK (FLAG) Affinity Gel can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).

Features

  • Specific—highly specific monoclonal antibody
  • High yield and high purity
  • Versatile—It can be used for protein purification or COIP
  • Convenient—reagents and peptides to elute are available separately

Applications

  • Immunoprecipitation (IP)
  • Purification of fusion proteins with Flag labels.


Specifications

Clone1E6
IsotypeMouse IgG2b
PurityProtein A
Antibody Concentration7.5 g antibody/L Gel
Protein binding capacity≥1.1 mg protein/mL Gel
Storage buffer10 mM Na3PO4, 150 mM NaCl, 50% glycerin, pH 7.4, contains 0.02% (w/v) sodium azide


Components

Components No.Name20585ES0120585ES0320585ES0820585ES2520585ES60
20585Anti-DYKDDDDK (Flag) Affinity Gel100 μL1 mL5 mL25 mL100 mL


Storage

The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!


Figures

  • The SDS-PAGE gel electrophoresis resultfromof Protein purification with YEASEN Anti-Flag Affinity Gel.

Figure 1. The SDS-PAGE gel electrophoresis results from Protein purification with YEASEN Anti-Flag Affinity Gel.

1: electrophoresis bands were purified by adding enterobacter lysate
2: negative control




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