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제품 설명
Rabbit IgG ELISA Kit
제품 번호
E-15G
제품 특징
Rabbit IgG ELISA Kit - For the quantitative determination of total IgG in biological samples.
The principle of the double antibody sandwich Rabbit IgG ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-Rabbit IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Rabbit IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of rabbit IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Product Size: 1.0 plate
More Information
More Information| Antibody Type | ELISA |
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| Host | Goat |
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| Target Species | Rabbit |
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| Specificity/Target | IgG h+l |
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| Size | 1.0 |
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| Detection Range | 7.81 ng/ml - 500 ng/ml |
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| Sensitivity | 1.920 ng/ml |
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| Assay Time | 70 min. |
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| Sample Type | Plasma, Serum |
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| Storage | 2-8C |
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Citiations
Zhang J, Jia G, Liu Q, et al. Silencing miR-146a influences B cells and ameliorates experimental autoimmune myasthenia gravis. Immunology. 2015;144(1):56-67. doi:10.1111/imm.12347
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