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제품 설명
Monkey IgG ELISA Kit
제품 번호
E-85E
제품 특징
The Monkey IgG ELISA kit is intended for the quantitative determination of total monkey IgG (new and old world) in biological samples.
The principle of the double antibody sandwich Monkey IgG ELISA is represented in Figure 1. In this assay the IgG present in samples reacts with the anti-Monkey IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, highly cross-adsorbed anti-Monkey IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of monkey IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Product Size: 1.0 plate
| Antibody Type | ELISA |
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| Target Species | Monkey |
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| Specificity/Target | IgG h+l |
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| Size | 1.0 |
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| Detection Range | 7.8 ng/ml - 500 ng/ml |
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| Sensitivity | 1.816 ng/ml |
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| Assay Time | 90 min. |
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| Sample Type | Plasma, Serum |
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| Storage | 2-8C |
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Citiations
Lovelace SE, Helmold Hait S, Yang ES, et al. Anti-viral efficacy of a next-generation CD4-binding site bNAb in SHIV-infected animals in the absence of anti-drug antibody responses. iScience. 2022;25(10):105067. Published 2022 Sep 5. doi:10.1016/j.isci.2022.105067
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