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제품 설명
ALCAR(Acetylcarnitine) ELISA Kit
제품 번호
EK247606
제품 특징
SKU: | EK247606 |
Category: | |
Species: | General |
Assay Principle: | Quantitative |
Assay Type: | Competitive Inhibition |
Target Name: | ALCAR(Acetylcarnitine) |
Sample Type: | serum, plasma and other biological fluids |
Sensitivity: | 25.2 ng/mL |
Standard: | 5000 ng/mL |
Detection Range: | 78.13-5000 ng/mL |
Assay Time: | 2h |
Research Area: | Metabolic pathway;Neuro science;Nutrition metabolism; |
시약 준비 과정
Standard Working Solution
First, centrifuge the Standard at 1000 x g for 1 minute. Then, reconstitute the Standard by adding 1.0 ml of Standard Diluent Buffer. Allow the mixture to sit for approximately 10 minutes at room temperature, and gently shake it (avoiding foaming).
Prepare 7 tubes with 0.5 ml of Standard Diluent Buffer in each and create a series of dilutions using the Diluted Standard. Remember to thoroughly mix each tube before transferring the solution to the next tube by pipetting the solution up and down several times. Set up 7 points of Diluted Standard, decreasing the concentration each time, and use a Blank with no standard for comparison. It's important to use a new Standard Solution for each experiment to ensure accurate results. When diluting the Standard from high concentration to low concentration, replace the pipette tip for each dilution. Additionally, make sure not to transfer solution into the Blank tube from the previous one.
Tube | 6 | 5 | 4 | 3 | 2 | 1 | 0 |
ng/mL | 5000 | 2500 | 1250 | 625 | 312.5 | 156.25 |
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