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제품 설명

Nitrite Quantification Assay Kit

제품 번호

CA-NO100

제품 특징

Detailed Description

About Elite™ Nitrite Quantification Assay Kit

Nitrite (NO₂⁻) is one of the primary stable oxidation products of nitric oxide (NO) metabolism and serves as a widely accepted indicator of nitric oxide production in biological systems. Nitric oxide is synthesized from L-arginine by nitric oxide synthases (NOS) and plays essential roles in vascular regulation, neurotransmission, immune defense, inflammation, and cell signaling. Because NO is highly reactive and has a very short biological half-life, direct measurement is often impractical. Instead, quantification of nitrite provides a reliable and convenient approach for monitoring nitric oxide production and evaluating NO-related physiological and pathological processes. Measurement of nitrite has become a standard technique in cardiovascular, immunology, neuroscience, inflammation, and cancer research.

The Elite™ Nitrite Quantification Assay Kit provides a rapid, sensitive, and convenient colorimetric assay for the quantitative determination of nitrite concentrations in biological samples. The assay utilizes the well-established Griess reaction, in which nitrite reacts with aromatic reagents under acidic conditions to produce a stable colored azo compound that can be measured at 540 nm using a standard microplate reader. The simple mix-and-read protocol requires minimal sample preparation and is suitable for analyzing cell culture supernatants, serum, plasma, urine, tissue extracts, and other biological fluids. Optimized for 96-well microplate formats, the kit delivers excellent sensitivity, reproducibility, and compatibility with both routine laboratory studies and high-throughput screening applications.

Applications

Biological Significance of Nitrite

Nitric oxide is a multifunctional signaling molecule involved in numerous physiological processes, including regulation of vascular tone, platelet aggregation, neurotransmission, host defense, and immune cell activation. Following its production, NO is rapidly oxidized to nitrite and nitrate, with nitrite serving as a sensitive biomarker for endogenous NO generation. During inflammatory responses, activation of inducible nitric oxide synthase (iNOS) in macrophages and other immune cells leads to elevated nitrite levels, reflecting increased nitric oxide production. Likewise, alterations in nitrite concentrations have been associated with cardiovascular diseases, septic shock, neurodegenerative disorders, diabetes, cancer, and chronic inflammatory conditions. Consequently, quantitative measurement of nitrite is widely used to investigate nitric oxide signaling pathways, evaluate disease progression, and assess the pharmacological effects of nitric oxide donors, NOS inhibitors, and anti-inflammatory therapeutics.

The Elite™ Nitrite Quantification Assay Kit offers researchers a reliable, sensitive, and reproducible platform for measuring nitrite levels across a wide variety of biological samples. Its straightforward workflow, robust colorimetric detection, and compatibility with standard laboratory instrumentation make it an ideal tool for studies in cardiovascular biology, immunology, neuroscience, toxicology, oxidative stress, and drug discovery. Whether monitoring nitric oxide production in cultured cells or evaluating nitrite concentrations in biological fluids, this assay delivers accurate and consistent results for diverse biomedical research applications.

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