AntibodySystem는 Primary Antibody Biosimilar - Research Grade 

제품을 전문적으로 생산하는 20년의 경험이 있는 High Quality 제조사입니다. 

제품 설명 

Golimumab ELISA Kit

제품 번호

KDB94403

제품 특징

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human TNFa has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Golimumab in the sample competitively binds to the pre-coated protein with biotin-labeled Golimumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Golimumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Catalog No.

KDB94403

Applications

Used for the quantitative determination of Golimumab concentration in serum and plasma.

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.

Detection method

Colorimetric

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

Sample type

Plasma, Serum

Assay type

Quantitative

Sensitivity

40.1 ng/mL

Range

62.5 - 4,000 ng/mL

Recovery

80-120%

Specifications

Golimumab

Alternative Names

CNTO 148, CAS: 476181-74-5

Background

Golimumab (Simponi®) is a human immunoglobulin G1? monoclonal antibody which is specific for pro-inflammatory cytokine, tumor necrosis factor-a (TNFa). In 2009, it was approved by FDA for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis in adult patients. Elevated levels of TNF are found in the synovial fluid of rheumatoid arthritis, including juvenile idiopathic arthritis, psoriatic arthritis, and ankylosing spondylitis patients and play an important role in both the pathologic inflammation and the joint destruction that are hallmarks of these diseases. Increased levels of TNF are also found in psoriasis (Ps) plaques. Golimumab binds to both the soluble and transmembrane bioactive forms of human TNF and prevent TNF from binding to its receptors and finally inhibits biological activity of TNF.

Shipping

2-8 ℃

Note

For Research Use Only.

AntibodySystem의 모든 제품들을 만나 보세요!  

Products

Recombinant Protein

Antibody

Assay Kit

Research Biosimilar

Enzyme

Trending Product

Super-Affinity Antibody

Cell Lysates

{ const originalImageSrc = iconImages[index].src; li.addEventListener('mouseenter', () => { iconImages[index].src = originalImageSrc.replace('.png', '_w.png'); }); li.addEventListener('mouseleave', () => { iconImages[index].src = originalImageSrc; }); }); function goBack(){ window.history.back(); }