CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체,
10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다.
제품 설명
Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit
제품 번호
CSB-E08854r
제품 특징
Precision
Intra-assay Precision (Precision within an assay): CV%<8% |
Three samples of known concentration were tested twenty times on one plate to assess. |
Inter-assay Precision (Precision between assays): CV%<10% |
Three samples of known concentration were tested in twenty assays to assess. |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample | Serum(n=4) | |
1:1 | Average % | 87 |
Range % | 80-95 | |
1:2 | Average % | 95 |
Range % | 85-99 | |
1:4 | Average % | 95 |
Range % | 86-102 | |
1:8 | Average % | 98 |
Range % | 82-104 |
Recovery
The recovery of rat BCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type | Average % Recovery | Range |
Serum (n=5) | 95 | 85-100 |
EDTA plasma (n=4) | 95 | 87-102 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml | OD1 | OD2 | Average | Corrected |
20 | 2.745 | 2.768 | 2.757 | 2.572 |
10 | 2.176 | 2.165 | 2.171 | 1.986 |
5 | 1.485 | 1.463 | 1.474 | 1.289 |
2.5 | 0.896 | 0.864 | 0.880 | 0.695 |
1.25 | 0.569 | 0.543 | 0.556 | 0.371 |
0.625 | 0.425 | 0.439 | 0.432 | 0.247 |
0.312 | 0.296 | 0.284 | 0.290 | 0.105 |
0 | 0.185 | 0.184 | 0.185 |