CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체,
10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다.
제품 설명
Mouse tumor necrosis factor α (TNF-α) antibody ELISA Kit
제품 번호
CSB-EQ023955MO
제품 특징
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<10% |
| Three samples of known concentration were tested in twenty assays to assess. |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample | Serum(n=4) | |
| 1:1 | Average % | 85 |
| Range % | 80-92 | |
| 1:2 | Average % | 97 |
| Range % | 90-105 | |
| 1:4 | Average % | 87-96 |
| Range % | 82-96 | |
| 1:8 | Average % | 93 |
| Range % | 88-100 |
Recovery
The recovery of mouse TNF-α antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
| Sample Type | Average % Recovery | Range |
| Serum (n=5) | 90 | 85-94 |
| EDTA plasma (n=4) | 92 | 87-96 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| ng/ml | OD1 | OD2 | Average | Corrected |
| 10 | 2.713 | 2.752 | 2.733 | 2.580 |
| 5 | 2.212 | 2.349 | 2.281 | 2.128 |
| 2.5 | 1.514 | 1.623 | 1.569 | 1.416 |
| 1.25 | 0.849 | 0.864 | 0.857 | 0.704 |
| 0.625 | 0.502 | 0.525 | 0.514 | 0.361 |
| 0.312 | 0.313 | 0.316 | 0.315 | 0.162 |
| 0.156 | 0.257 | 0.270 | 0.264 | 0.111 |
| 0 | 0.148 | 0.157 | 0.153 |
MSDS
온라인 견적