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제품 설명
GENEzol™ Reagent
제품 번호
GZR050, GZR100, GZR200
제품 특징
Introduction
RNA Extraction/Tri RNA
GENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting. GENEzol™ Reagent is a cost effective alternative for efficient total RNA extraction.
Description
Advantages (Cat. # GZR050, GZR100, GZR200)
- Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
- Sample Volume: up to 300 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 50-100 mg of tissue
- A cost effective, scalable solution
- Format: phenol, chlorform, guanidine isothiocyanate
- Storage: dry at 2ºC to 25ºC
APPLICATIONS
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
COMPONENTS
QUALITY CONTROL
GENEzol™ Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. RNA from a 1 ml human blood sample is extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.
GENEzol™ Reagent Comparison Data
Figure 1 .Bacterial RNA was extracted from 5 x 108 E. coli cells using our top competitor's TRI reagent and GENEzol™ Reagent. The E. coli cells were incubated with lysozyme for 5 minutes at room temperature. 1 ml of reagent was then added to the cell lysate. The RNA pellets were dissolved in 50 μl of RNase-free water.
TRI Reagent = 1, 2
GENEzol™ Reagent = 3, 4
Sample
ng/µl
260/280
260/230
Yield
GENEzol™ Reagent Functional Test Data
Figure 2 .RNA from a 1 ml human blood sample was extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder
Sample
RNA Conc.
260/280
Yield
GENEzol™ Reagent real-time PCR Data
MSDS
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