(주)모아바이오

Geneaid

[Geneaid] EcoExtra™ Magnetic Beads Plasmid Kit

Cat-No. MPD192, MPD576, MPD1920

Geneaid는 분자 생물학 연구와 진단을 위한 고품질 핵산 추출 및 분석 솔루션을 제공하는 글로벌 생명과학 기업입니다.

제품 설명

EcoExtra™ Magnetic Beads Plasmid Kit

제품 번호

MPD192, MPD576, MPD1920

제품 특징

Introduction

DNA Extraction/Plasmid DNA Purification

The EcoExtra™ Magnetic Beads Plasmid Kit was designed for high throughput purification of plasmid DNA from up to 16 ml of cultured bacterial cells per reaction using an efficient magnetic beads system. A modified alkaline lysis method and RNase treatment a re used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 6-10 μ g for high copy number plasmid from 1 ml of cultured E. coli cells. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions. More important, the EcoExtra™ series kits enable up to 80% reduction of plastic material waste compared to silica-column based kits. Make lab works a lot more eco friendly.

Description

Specifications (Cat. # MPD192, MPD576, MPD1920)

Sample Volume: 1-16 ml of cultured bacterial cells
High Yield: 6-10 μg for high-copy number plasmid from 1 ml of cultured E. coli cells
High Purity plasmid DNA: A260/A280 =1.8-2.0, A260/230 =2.0-2.4
Plasmid DNA Size: 1-20 kb
Elution volume: 25-100 µl
Manual or automated plasmid DNA isolation
Storage: dry at room temperature (15-25°C)

APPLICATIONS

Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions

COMPONENTS

MPD1 Buffer
MPD2 Buffer
MPD3 Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
RNase A
MPD Magnetic Beads

QUALITY CONTROL

The quality of the EcoExtra™ Magnetic Beads Plasmid Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript is purified using the EcoExtra™ Magnetic Beads Plasmid Kit . Following the purification process, a yield of more than 30 μg is obtained and the A260/A280 ratio is between 1.8-2.0.

EcoExtra™ Magnetic Beads Plasmid Kit Functional Test Data

Figure 1 .Plasmid DNA was extracted using the EcoExtra™ Magnetic Beads Plasmid Kit. 5 µl aliquots of a 100 µl eluate of purified super coiled plasmid DNA from 1, 2, and 4 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (OD600 = 4 ). The purified plasmid were analyzed by electrophoresis on a 0.8% agarose gel.

Lane M = Geneaid 1 Kb DNA Ladder, Lane1-2 =1 ml, Lane 3-4 =2 ml, Lane 5-6 =4 ml cultured E. coli cells

Sample

Yield

260/280

260/230

1ml E.coli cells

6-10 µg

1.8-2.0

2.0-2.4

2ml E.coli cells

15-20 µg

1.8-2.0