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YEASEN

[YEASEN] GoldBand™ Plus 3-color Regular Range Protein Marker(8-180 kDa)

Cat-No. 20350ES
20350ES72 / size: 25μL
20350ES76 / size: 2x250μL
20350ES90 / size: 10x250μL

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.




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GoldBand™ Plus 3-color Regular Range Protein Marker(8-180 kDa)




제품 번호


20350ES72 / size: 25μL

20350ES76 / size: 2x250μL

20350ES90 / size: 10x250μL

 



제품 설명


Description

This product consists of 10 highly purified and prestained proteins ranging in molecular weight from 8 kDa to 180 kDa (8,17,25,33,43,55,72,100,130,180kDa), among them, 72 kDa is orange band, 8 kDa is green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!
After the prestained protein is combined with the dye, the molecular weight will change in the gel of different concentrations. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein.

Features

  • Bright color - three color predye, each strip average concentration of 2.5 μg
  • Lower amount of this product into the gel hole - mini-gel: 3-5 μL; 
  • Stable performance - 50℃ for 20 h, no degradation
  • Long shelf life - can be stored at -15℃ ~ -25℃ for two years

Applications

  • Protein migration is monitored during SDS-PAGE electrophoresis
  • Monitor protein transfer to the membrane after Western blotting
  • Protein size analysis on SDS-PAGE and Western blotting


Specifications

Product Type SpecsProtein Ladder
Size Range8 kDa to 180 kDa
Number of Markers10
Molecular Weight8,17,25,33, 43,55,72,100,130,180 kDa
Stain Type72 kDa is orange band, 8 kDa is green band, the rest are blue bands
Storage Buffer62.5 mM Tris-H3PO4(pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300
Ready to Loadready-to-use product, do not heat, dilute or add reducing agents


Components

Components No.Name20350ES7220350ES7620350ES90
20350GoldBand™ Plus 3-color Regular Range Protein Marker (8-180 kDa)250 μL2×250 μL10×250 μL


Storage

The product should be stored at -25℃ ~ -15℃ for two years. For regular use, it can be placed at 4℃ for short time use. It is recommended to store in aliquots to avoid repeated freezing and thawing!


Figures

Figure 1 12% SDS-PAGE electrophoresis results

 

Figure 2 Under different electrophoresis buffer conditions, the position each band of this product




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