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제품 설명 

EnzyChrom™ NAD/NADH Assay Kit

제품 번호

E2ND-100

제품 특징

Application

• For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.

Key Features

• Sensitive and accurate. The detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• OD565nm

Samples

• Cell or tissue extracts

Species

• All

Procedure

• 15 min

Size

• 100 tests

Detection Limit

• 0.05 µM

Shelf Life

• 6 months

More Details

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD⁺/NADH has applications in research pertaining to energy transformation and the redox state of cells or tissue. Simple, direct, and automation-ready procedures for measuring NAD⁺/NADH concentration are very desirable. BioAssay Systems EnzyChrom™ NAD⁺/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD⁺/NADH concentration in the sample. This assay is highly specific for NAD⁺/NADH and with minimal interference (<1%) by NADP⁺/NADPH. Our assay is a convenient method to measure NAD, NADH, and their ratio.

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