본문 바로가기 주메뉴 바로가기

제품소개

Home

제품상세페이지

Cusabio

[Cusabio] Rat Caspase 3,Casp-3 ELISA Kit

Cat-No. CSB-E08857r


CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체, 

10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다. 



제품 설명


Rat Caspase 3,Casp-3 ELISA Kit



제품 번호


CSB-E08857r



제품 특징


Product Details

Target Name
caspase 3, apoptosis-related cysteine peptidase
Alternative Names
Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; IRP ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich


Precision

To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


Sample		Serum(n=4) 
1:1Average %84
Range %80-92
1:2Average %98
Range %91-105
1:4Average %99
Range %92-110
1:8Average %91
Range %86-98


Recovery

The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample TypeAverage % RecoveryRange
Serum (n=5)9589-98
EDTA plasma (n=4)9690-100


Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2AverageCorrected
202.9152.9032.9092.828
102.3212.2832.3022.221
51.4671.4441.4561.375
2.50.7270.7060.7170.636
1.250.3650.3590.3620.281
0.6250.2160.2060.2110.130
0.3120.1370.1290.1330.052
00.0840.0780.081


Materials provided

  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CASP3 antibody.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled CASP3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) -
목록