Catalog#： YC-C083-Luc-P
Size： 1*10^6
Culture method： 90%RPMI-1640+10% FBS
Detailed Product Information
| Product category | Luciferase Stable Cell Lines | Mycoplasma test | negative |
| Cell Morphology | Lymphoblast,suspension | Culture method | 90%RPMI-1640+10% FBS |
| Passage ratio | 1：2-1：4 | Species | Human |
| Reporter gene | Luciferase | Resistance gene | puro |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Description
Luciferase is the general name of a class of enzymes that catalyze the oxidation and luminescence of luciferin or fatty aldehydes in organisms. Currently, firefly luciferase is the most commonly used enzyme in scientific research. This enzyme can catalyze the oxidative decarboxylation of D2 luciferin, produce activated oxidized luciferin, and emit reddish biological fluorescence. Because luciferase is based on the mutual reaction between enzyme and substrate, it has the characteristics of strong specificity, high sensitivity and signal-to-noise ratio, as well as strong penetration ability of biological fluorescent tissue. Ubigene's KG-1a-Luc cell line was constructed on the basis of wild-type KG-1a cells by lentiviral transduction, which can stably express firefly luciferase for a long time. This cell line has low passage, high activity and good condition, and can be directly used for in vivo cell injection, which can well help you with the research of transcription factor regulation mechanism, in vivo imaging, cell tracking and other experiments.
Imaging process of stable tumor cell lines in vivo
STR Profile| Loci | STR Info (Sample Cell)
Sample Cell Line:KG-1a | STR Info (Cell bank)
Cell Line:KG-1 |
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 |
| D5S818 | 13 | 13 |
| 13 | 13 |
|
| D13S317 | 11 | 12 |
| 11 | 12 |
|
| D7S820 | 8 | 10 |
| 8 | 10 |
|
| D16S539 | 10 | 11 |
| 10 | 11 |
|
| VWA | 14 | 19 |
| 14 | 19 |
|
| TH01 | 7 | 8 |
| 7 | 8 |
|
| AMEL | X | Y |
| X | Y |
|
| TPOX | 7 | 9 |
| 7 | 9 |
|
| CSF1PO | 7 | 7 |
| 7 | 7 |
|
| D12S391 | 21 | 21 |
|
|
|
|
| FGA | 22 | 22 |
|
|
|
|
| D2S1338 | 20 | 23 |
|
|
|
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| D21S11 | 28 | 29 |
|
|
|
|
| D18S51 | 10.2 | 18 |
|
|
|
|
| D8S1179 | 13 | 14 |
|
|
|
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| D3S1358 | 15 | 16 |
|
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| D6S1043 | 18 | 19 |
|
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| PENTAE | 7 | 12 |
|
|
|
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| D19S433 | 16.2 | 16.2 |
|
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| PENTAD | 8 | 9 |
|
|
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| D1S1656 | 13 | 14 | 16.3 |
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* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Product Advantage
Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
MSDS
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