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YEASEN

[YEASEN] Hieff Clone™ Universal One Step Cloning Kit

Cat-No. 10922ES

10922ES20 / size: 20T
10922ES50 / size: 50T

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제품 설명

Hieff Clone™ Universal One Step Cloning Kit

제품 번호

10922ES20 / size: 20T

10922ES50 / size: 50T

제품 설명

Description

Hieff Clone™ Universal One Step Cloning Kit is a simple, fast, and highly efficient cloning technology and enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively, are mixed in an appropriate ratio and incubated with recombinase at 50℃ for 5-15 min.

Hieff Clone™ Universal one step cloning kit is a novel cloning Kit, independent of DNA ligase, significantly reducing the self-ligated colonies and bringing a true positive rate >95%.

Features

Simple: Seamlessly assemble and clone up to six DNA fragments in a single reaction.
Flexible: Design guidelines allow assembly into any vector of your choice.
Efficient: Efficient for ligation of one to six fragments.

Application

Quick Clone: It allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Specifications

Cloning Process	Seamless cloning
Control	Positive control
Segments	Up to 6 fragments
Product Type	Seamless Cloning and Assembly Kit

Components

Components No.	Name	10922ES20 (20T)
10922-A	Hieff Clone™ Universal Enzyme Premix	200 μL
10922-B	500 bp Control Insert (25 ng/μL)	5 μL
10922-C	pUC 19 Control Vector, linearized (50 ng/μL)	5 μL

Storage

The Hieff Clone™ Universal One Step Cloning Kit should be stored at -25℃~ -15°C for 1 year.

Figures

Figure 1 Multi-segment linkage: performance comparison with competitive strains (plate colony).

Top half of the picture: 5 fragments+vector; Total segment length: 4000 bp; Vector length: 5000 bp
Bottom half of the picture: 6 fragments+vector; Total segment length: 5000 bp; Vector length: 5000 bp

Figure 2 Multi-segment linkage: performance comparison with competitive strains (colony PCR).

Top half of the picture: 5 fragments+vector; Total segment length: 4000 bp; Vector length: 5000 bp
Bottom half of the picture: 6 fragments+vector; Total segment length: 5000 bp; Vector length: 5000 bp

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